Inosine

Inosine, an endogenous purine nucleoside, has immunomodulatory, neuroprotective, and analgesic properties. In vitro: Inosine has been shown to stimulate axonal growth in cell culture and promote corticospinal tract axons to sprout collateral branches after stroke, spinal cord injury and TBI in rodent models.[1] Inosine dose-dependently stimulates cAMP production mediated through the A2AR. Inosine dose-dependently induces A2AR-mediated ERK1/2 phosphorylation.[2] In vivo: The reference for

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Inosine is a purine nucleoside in which hypoxanthine is attached to ribofuranose via a beta-N(9)-glycosidic bond. It has a role as a human metabolite, a Saccharomyces cerevisiae metabolite, an Escherichia coli metabolite and a mouse metabolite. It is a purines D-ribonucleoside and a member of inosines. It derives from a hypoxanthine and a ribofuranose.|A purine nucleoside that has hypoxanthine linked by the N9 nitrogen to the C1 carbon of ribose. It is an intermediate in the degradation of purines and purine nucleosides to uric acid and in pathways of purine salvage. It also occurs in the anticodon of certain transfer RNA molecules. (Dorland, 28th ed)

Method 1: Preparation of inosinate disodium inosinate dephosphorylation Corynebacterium 269 is fermented, the fermentation broth is decellularized, adsorbed, eluted, concentrated, added with ethanol, and crystallized under pH 7-7.5 to obtain disodium inosinate . Corynebacterium 269 [fermentation] → fermentation broth [debacteria, adsorption, elution, concentration] → concentrated solution [ethanol] → [pH7-7.5] Preparation of crude inosine disodium inosinate in acetic acid buffer, adjusted pH5.4-5.6, pressurized 137-147kPa, dephosphorylation for 5h, can get crude inosine. Disodium inosinate [acetic acid, pressurized] → dissolved inosine purified distilled water, pH=6, recrystallized to obtain high-quality products. Method two: One-step fermentation strain selection Bacillus mutans 7171-9-1 was transferred to slant medium and cultured at 30-32℃ for 48h. The components of the slant medium are glucose 1%, peptone 0.4%, yeast extract 0.7%, beef extract 1.4%, agar 2%, and sterilized at pH 7, 120°C for 20 min. Bacillus mutans 7171-9-1[Bevel culture]→[30-32℃, 48h] Bacterial seed bevel seed culture first-level seed: medium composition is glucose 2%, peptone 1%, yeast extract 1%, corn steep liquor 0.5%, urea 0.5%, sodium chloride 0.25%. Before sterilization, the pH was 7, and 150ml of medium was filled in a 1L triangular bottle and sterilized at 115°C for 15min. Put platinum earring fungus into each Erlenmeyer flask and incubate on a reciprocating shaker at 32℃±1℃ for 18h. Second-level seed: medium-level seed, enlarged 50L fermentor, fixed volume 25L, inoculation volume 3%, culture at 32℃±1℃ for 12-15h, stirring speed 320r/min, ventilation volume 1: 0.25V/(V ·Min), growth bacteria index A(650nm)=0.78, pH=6.4-6.6. Bacterial slant [shaker shaking]→[pH7, 32℃±1℃, 18h] first-level seed culture [50L fermentation tank]→[pH6.4-6.6, 32℃±1℃, 12-15h] second-level The seed culture broth is fermented in a 50L stainless steel standard fermenter with a fixed volume of 35L. The medium composition is starch hydrolyzed sugar 10%, dry yeast hydrolysate 1.5%, soybean cake hydrolysate 0.5%, urea 0.4%, magnesium sulfate 0.1%, potassium chloride 0.2%, disodium hydrogen phosphate 0.5%, ammonium sulfate 1.5%, Silicone oil 0.05%. pH=7, inoculation volume 0.9%, incubation at 32℃±1℃ for 93h, stirring speed 320r/min, ventilation volume 1: 0.5V/(V·min). 500L fermentation tank, constant volume 350L. The medium composition is starch hydrolyzed sugar 10%, dry yeast hydrolysate 1.5%, soybean cake hydrolysate 0.5%, ammonium sulfate 1.5%, magnesium sulfate 0.1%, potassium chloride 0.2%, disodium hydrogen phosphate 0.5%, calcium carbonate 1% , Silicone oil is less than 0.3%. pH=7, inoculation volume 7%, culture at 32℃±1℃ for 75h, stirring speed 230r/min, ventilation volume 1: 0.25V/(V·min). 20000L fermentor, the culture medium is the same as above, the inoculation volume is 2.5%, and the culture is 35℃±5℃ for 83h. Secondary seed culture [50L fermentation tank]→[pH7, 32℃±1℃, 93h] fermentation broth[500L fermentation tank]→[pH7, 32℃±1℃, 75h] fermentation broth[20000L fermentation tank]→[ pH7, 35℃±1℃, 83h] fermentation broth extraction, adsorption, elution The fermentation broth 30-40L was adjusted to pH=2.5-3, and the bacteria were adsorbed by two 3.5kg 732H resin columns connected in series. Wash once with pH 3 water equivalent to 3 times the total volume of the resin, separate the two columns, elute the inosine with pH 3 water, absorb the inosine through the 769 activated carbon column, and wash with 2-3 times the volume of water , Then wash with 70-80℃, 70-80℃, soak in 1mol/L sodium hydroxide solution for 30min, and finally elute inosine with 0.01mol/L sodium hydroxide solution, collect the eluent, concentrate in vacuum, pH11 or 6 Place and filter to get crude product. Fermentation broth [732 resin column] → [pH2.5-3] adsorbate [water] → [pH2.5-3] eluent [769 activated carbon column] → adsorbate [distilled water, sodium hydroxide] → [70- 80℃][Sodium hydroxide]→[70-80℃] Eluent [Concentrated crystal]→[pH11 or 6]Purification of crude inosine The crude product is formulated into 5%-10% solution, heated to dissolve, suction filtered, After cooling, filtering, washing with a small amount of water, and drying, the inosine refined product is obtained. Crude inosine [distilled water] → [recrystallization] finished inosine.

cell function activator, cardiotonic.Participate in energy metabolism and protein synthesis in the body.Used as coenzyme drugs.Biochemical research. Medicine is used to promote the growth of white blood cells.Inosine is a potential stimulator of nerve production factor (NGF).

Molecular Weight:268.23
XLogP3:-1.3
Hydrogen Bond Donor Count:4
Hydrogen Bond Acceptor Count:7
Rotatable Bond Count:2
Exact Mass:268.08076950
Monoisotopic Mass:268.08076950
Topological Polar Surface Area:129
Heavy Atom Count:19
Complexity:405
Defined Atom Stereocenter Count:4
Covalently-Bonded Unit Count:1
Compound Is Canonicalized:Yes