There are three types of chemiluminescence in nature: physical luminescence, chemiluminescence and bioluminescence,In a narrow sense, chemiluminescence immunoassay mainly refers to chemiluminescence. Chemiluminescence is a kind of oxidation chemical reaction. After electron excitation, it returns to the ground state and releases energy in the form of photons. The number of photons released reflects the intensity of chemical reaction. The commonly used luminescent agents are aminophenylenedihydrazide (isoluminol and luminol) and acridine esters.
Chemiluminescence immunoassay (CLIA) is a non radioactive immunoassay developed rapidly in recent 30 years, which belongs to a kind of ultra-high sensitivity microanalysis technology. It combines chemiluminescent system with immune reaction, labels antibody or antigen with chemiluminescent related substances, reacts with antigen or antibody to be tested, separates free state chemiluminescent markers, and adds other related substances of chemiluminescent system to produce chemiluminescent, and carries out quantitative or qualitative detection of antigen or antibody.
The advantages of chemiluminescence immunoassay are as follows
(1) high sensitivity: theoretically, the highest sensitivity of chemiluminescence can reach 10-18mol / L.
(2) short detection time: the time for measuring the optical signal of each sample is not more than a few seconds, and it can be completed within 9-60 minutes from sample addition to analysis results.
(3) wide detection range: theoretically, the maximum linear range can reach 105 relative luminous units (RLU), namely 5 orders of magnitude.
(4) the analytical method is simple and rapid: most of the analytical methods only need to add one reagent (or compound reagent).
(5) high sensitivity: higher than RIA.
(6) good safety and long service life: avoid the use of radioactive materials. So far, no harm has been found; the reagent is stable and can be stored for more than six months to one year.
The disadvantages of chemiluminescence immunoassay are as follows
(1) The luminescent process is short
(2) high background
(3) instrument failure rate is high
(4) the stability of the reagent is poor
(5) the detection accuracy is not high
The application of chemiluminescence immunoassay (CLIA)
Chemiluminescent immunoassay (CLIA) has unique characteristics of high sensitivity, high specificity, rapidity, accuracy, specificity and automaticity. It plays an important role in clinical examination, drug analysis, environmental monitoring and other fields. Especially the determination of hormones, tumor markers, drug concentrations and other trace bioactive substances.
The clinical application of chemiluminescence immunoassay is mainly reflected in the treatment of thyroid hormone, adrenal / pituitary hormone, anemia factor, tumor markers, infectious diseases, allergic diseases, therapeutic drug monitoring and so on, which ensures the stability and reliability of data results. The detection of tumor markers has a certain application value in the early detection of tumor, the development of the disease, the evaluation after treatment, and the monitoring of recurrence and metastasis.
Desheng biotechnology has been making chemiluminescent reagents for many years. In addition to chemiluminescent reagents, there are other series of products such as vascular additives, biological buffers, chromogen substrates, etc. chemiluminescent reagents are products developed and produced by Desheng biotechnology for many years, which can fully meet the requirements of various manufacturers with reliable quality and favorable price. If you are interested, you can call to talk about them in detail.
