HEPES, 4-hydroxyethylpiperazine ethanesulfonic acid, is a commonly used biological buffer, usually used in cell preservation solutions, DNA/RNA extraction kit, PCR diagnostic kit. According to different needs, HEPES can be configured into a variety of different buffer systems.
HEPES buffer has many advantages, good buffering performance, non-toxic effect on cells, it is a hydrogen ion buffer,It can control the system in a constant pH range for a long time. Its pH buffer range is 6.8-8.2, and the final concentration is 10-50mmol/L, generally the buffering capacity can be achieved when the culture medium contains 20mmol/L HEPES.
According to the specific requirements of different experiments, the composition of the HEPESbuffer buffer and the preparation method are also different.
Usually HEPES is combined with sodium hydroxide NaOH or HEPES+ salt.
1. When preparing HEPES Stocksolution, first weigh HEPES and dissolve it in distilled water, and then use 0.5-1M To adjust the pH with NaOH, note that the effective pH buffer range is 6.8-8.2, then distilled water to a constant volume and store at 4°C.
2. When preparing HEPES buffer salt solution, you need to add sodium chloride, potassium chloride, disodium hydrogen phosphate, dextran, etc.Use NaOH to adjust the pH of the sample, and finally store it at a constant volume at a low temperature.
3. LeageneHEPES buffer, a commonly used solution in cell-cell adhesion experiments, is mainly composed of HEPES and chlorinated It is composed of sodium, potassium chloride, phosphate, glucose, etc., and contains Ca2+ and Mg2+ ions, pH 7.4, filtered and sterilized. Often It is used to clean tissues or cells (especially embryonic cells) in the process of long-term aggregation culture to protect the cadherin of the cells, and at the same time Does not affect the formation of cell aggregation.
4. HEPES-BSA solution, referred to as HA solution for short, does not contain calcium and magnesium ions, and is one of the components of cell culture fluid. Containing some other salt ions, after filtering and sterilizing treatment, it is mostly suitable for washing and culturing tissue cells in primary culture. In addition to the above buffers, HEPES can also be configured into many buffer systems, such as cell culture medium, Hanks Both the preservation solution and the virus preservation solution contain HEPES. Desheng is a manufacturer specializing in the production of HEPES. The raw material is reagent powder. Different buffers can be configured according to requirements.
