Manufacture Supply 99 Bovine Serum Albumin CAS 9048-46-8

BSA is generally used as a stabilizer in storage solutions and reaction solutions for restriction enzymes or modified enzymes, because some enzymes are unstable or have low activity at low concentrations. After adding BSA, it may act as a "protector" or "carrier", and many enzymes can greatly increase their activity after adding BSA. Enzymes that do not need to add BSA will generally not be affected by adding BSA. For most substrate DNA, BSA can make the digestion more complete, and can achieve repeated cutting. At 37ºC, when the digestion reaction exceeds 1h, BSA can make the enzyme more stable, because in the reaction buffer without BSA, many restriction enzymes can only survive for 10"20min or even shorter time at 37ºC And BSA can bind to metal ions and other chemicals in the buffer or substrate DNA that inhibit the activity of restriction enzymes.