Native Bacillus stearothermophilus Leucine Dehydrogenase
9082-71-7
Yes
2026-11-14
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Product Description
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Seller Information
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Description
ProductName Native Bacillus stearothermophilus Leucine Dehydrogenase Cat No NATE-1905 Description In enzymology, a leucine dehydrogenase (EC 1.4.1.9) is an enzyme that catalyzes the chemical reaction: L-leucine + H2O + NAD+ ↔ 4-methyl-2-oxopentanoate + NH3 + NADH + H+. The 3 substrates of this enzyme are L-leucine, H2O, and NAD+, whereas its 4 products are 4-methyl-2-oxopentanoate, NH3, NADH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH2 group of donors with NAD+ or NADP+ as acceptor. This enzyme participates in valine, leucine and isoleucine degradation and valine, leucine and isoleucine biosynthesis. Abbr LeuDH, Native (Bacillus stearothermophilus) Alias LeuDH Source Bacillus stearothermophilus Applications The enzyme is useful for determination of L-leucine, L-valine or L-isoleucine. Appearance Lyophilized Enzyme Commission Number EC 1.4.1.9 CAS No 9082-71-7 Contaminants (as LeuDH activity = 100 %)NADH oxidase: < 0.01 %;Lactate dehydrogenase: < 0.01 %. Molecular Weight ca. 300,000; Subunit molecular weight : ca. 49,000. pH Stability 6.0 - 11.5 Michaelis Constant (125mM Sodium phosphate buffer, pH 10.5, at 30 °C)L-Leucine: 3.4 mM;NAD+: 0.3 mM. Specificity L-Leucine: 100 %;L-Valine : 86 %;L-Isoleucine: 73 %. Unit Definition One unit of activity is defined as the amount of LeuDH that forms 1 μmol of NADH per minute at 30 °C Optimum pH 10.6 Thermal stability No detectable decrease in activity up to 60 °C. Storage Stable at -20 °C for at least one year. Synonyms EC 1.4.1.9; Leucine dehydrogenase; L-leucine: NAD+ oxidoreductase (deaminating); L-leucine dehydrogenase; L-leucine: NAD+ oxidoreductase (deaminating); LeuDH Specific Activity more than 40 U/mg protein Reaction L-Leucine + NAD+ + H2O ←→ α-Ketoisocaproate+ NH4+ +NADH category Amino Acids and Derivatives(c1517) cas_num 9082-71-7 ECHEMI Editorial ReferencePowderBasic Info-
Product Name:
Leucine dehydrogenase
Other Name:Leucine dehydrogenase;Dehydrogenase,leucine;E.C. 1.4.1.9;L-Leucine dehydrogenase;EC 1.4.1.9;Valine/leucine dehydrogenase;L-Leucine dehydrogenases;Valine/leucine dehydrogenases;Leucine dehydrogenases;L-Leucine dehydrogenases;L-Leucine dehydrogenase;1: PN: CN108103038 SEQID: 3 claimed sequence
CAS No.:9082-71-7
HScode:35079090
Categories:
Characteristics-
Appearance:
slightly brown solution
Storage Conditions:−20°C
Hazard IdentificationClassification of the substance or mixture
no data available
GHS label elements, including precautionary statements
Pictogram(s) no data available Signal word no data available
Hazard statement(s) no data available
Precautionary statement(s) Prevention no data available
Response no data available
Storage no data available
Disposal no data available
Other hazards which do not result in classification
no data available
Handling and StoragePrecautions for safe handling
Handling in a well ventilated place. Wear suitable protective clothing. Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Use non-sparking tools. Prevent fire caused by electrostatic discharge steam.
Conditions for safe storage, including any incompatibilities
Store the container tightly closed in a dry, cool and well-ventilated place. Store apart from foodstuff containers or incompatible materials.
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Seller Information
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Business Type:
Manufactory
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Main Products:
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Location:
Shirley, New York 11967, USA
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Payment Terms:
TT against copy of documents,D/P
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Average lead Time:
15 days
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Total Annual Revenue:
$1 million-$2.5 million
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Year of Establishment:
2004
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