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Native Bovine Alkaline Phosphatase
Native Bovine Alkaline Phosphatase
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CAS No.:
9001-78-9
Price valid (until):
2026-09-14
Company Type:
Manufactory
Location:
United States
Qualification:
Main Products:
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Product Description
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Seller Information
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Description
ProductName Native Bovine Alkaline Phosphatase Cat No NATE-0053 Description Alkaline phosphatase (ALP, ALKP, ALPase, Alk Phos) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Abbr ALP, Native (Bovine) Alias ALP; ALKP Source Bovine intestinal mucosa Species Bovine Applications Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase. Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and hist ochemical detection. It is routinely used to dephosphorylate proteins and nucleic acids. It may be used for protein labeling when high sensitivity is required. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase. This product has been used to study the mon oclonal alkaline phosphatase-anti-alkaline phosphatase (APAAP) complex. High specific activity grade recommended for antibody and protein conjugation. Product Overview Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity. Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer. Form Type I, lyophilized powder; Type II, aqueous solution, solution in 3.2 M ammonium sulfate, 1 mM MgCl2 and 0.1 mM ZnCl2, pH 7.0; Type III, buffered aqueous solution, Solution in 3.0 M NaCl containing 5 mM MgCl2, 0.2 mM ZnCl2, and 30 mM triethanolamine, pH 7.6; Type IV, Type V, Type VI, buffered aqueous glycerol solution, Solution in 50% glycerol containing 5 mM Tris, 5 mM MgCl2 and 0.1 mM ZnCl2, pH 7.0. Enzyme Commission Number EC 3.1.3.1 Activity Type I, > 10 DEA units/mg solid; Type II, > 2 ,000 DEA units/mg protein; Type III, 2 ,000-4 ,000 DEA units/mg protein; Type IV, > 5,500 DEA units/mg protein; Type V, > 6,500 DEA units/mg protein; Type VI, > 4 ,000 DEA units/mg protein. CAS No 9001-78-9 Molecular Weight dimer mol wt ~160 kDa Unit Definition One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37°C. (One glycine unit is equivalent to ~3 DEA units) Storage 2-8°C Warnings Package sizes are based on DEA units Pathway Endochondral Ossification, organism-specific biosystem (from WikiPathways) Folate biosynthesis, organism-specific biosystem (from KEGG) Folate biosynthesis, conserved biosystem (from KEGG) Metabolic pathways, organism-specific biosystem (from KEGG) TNF-alpha NF-kB Signaling Pathway, organism-specific biosystem (from WikiPathways) Function The peri-partum characteristics of serum bone-specific alkaline phosphatase (BAP) and urinary deoxypyridinoline (DPD) in dairy cattle are reported. Results indicate that the presence of glycosylphosphatidylinositol increases the stability of alkaline phosphatase against chemical denaturation while it decreases its refolding yield by the artificial chaperone refolding technique. Reliable and reproducible estimates of k (cat) and K (m) from only two or three progress curves were obtained using alkaline phosphatase. GPI-anchored proteins are localized on the outer layer of plasma membranes and clustered in microdomains generally called lipid rafts. Synonyms Alkaline phosphatase; ALP; ALKP; ALPase; Alk Phos; EC 3.1.3.1; Alkaline phosphomonoesterase; Glycerophosphatase; Phosphomonoesterase category Other Chemical Drugs(c1420) cas_num 9001-78-9 ECHEMI Editorial ReferencesolidBasic Info-
Product Name:
Alkaline phosphatase
Other Name:Alkaline phosphatase;Phosphatase,alkaline;E.C. 3.1.3.1;Alkaline phosphomonoesterase;Alkaline phosphohydrolase;EC 3.1.3.1;Alkaline phosphatases;Alkaline phenyl phosphatase;AIP;Ostase;ALP;bIAP;RESCAP;Alkaline phosphomonoesterases;Alkaline phosphohydrolases;119940-17-9;131754-85-3
CAS No.:9001-78-9
InChIKeys:ITZMJCSORYKOSI-AJNGGQMLSA-N
Molecular Weight:496.56054
Exact Mass:496.276
EC Number:232-631-4
HScode:35079090
Categories:
Characteristics-
PSA:
227
XLogP3:-5
Appearance:white suspension
Density:1.12 g/mL at 20 °C
Water Solubility:It is soluble in water.
Storage Conditions:2-8°C
Hazard IdentificationClassification of the substance or mixture
Not classified.
GHS label elements, including precautionary statements
Pictogram(s) No symbol. Signal word No signal word
Hazard statement(s) none
Precautionary statement(s) Prevention none
Response none
Storage none
Disposal none
Other hazards which do not result in classification
no data available
Handling and StoragePrecautions for safe handling
Handling in a well ventilated place. Wear suitable protective clothing. Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Use non-sparking tools. Prevent fire caused by electrostatic discharge steam.
Conditions for safe storage, including any incompatibilities
Store the container tightly closed in a dry, cool and well-ventilated place. Store apart from foodstuff containers or incompatible materials.
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Seller Information
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Business Type:
Manufactory
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Main Products:
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Location:
Shirley, New York 11967, USA
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Payment Terms:
TT against copy of documents,D/P
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Average lead Time:
15
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Total Annual Revenue:
$1 million-$2.5 million
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Year of Establishment:
2004
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