Native Tritirachium album Proteinase K
39450-01-6
2026-09-26
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Product Description
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Seller Information
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Description
ProductName Native Tritirachium album Proteinase K Cat No NATE-0221 Description Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments:pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA. Abbr Endopeptidase K, Native (Tritirachium album) Alias proteinase K Source Tritirachium album Applications Proteinase K is useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. It is used for the removal of endotoxins bound to cationic proteins such as lysozyme and ribonuclease A. It is also useful for the isolation of hepatic, yeast, and mung bean mitochondria and is used to determine enzyme localization on membranes. Furthermore, it is used for the treatment of paraffin embedded tissue sections to expose antigen binding sites and for digestion of proteins from brain tissue samples. The product has been used to study its pre-treatment effects on the silk fibroin. The aspects analysed in this study included the crystallographic properties of hydroxyapatite (HAp), and the microstructure and microhardness of the composites. The enzyme has also been used to facilitate the access of probes to rRNA using FISH techniques to detect pathogenic Staphylococcus aureus. Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A. Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria Determination of enzyme localization on membranes Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling. Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. Form lyophilized powder or buffered aqueous glycerol solution Enzyme Commission Number EC 3.4.21.64 Activity 3.0-15.0 unit/mg solid; > 30 units/mg protein, ; > 800 units/mL; > 500 units/mL CAS No 39450-01-6 Molecular Weight 28.93 kDa Unit Definition One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37°C. Synonyms Tritirachium alkaline proteinase; Tritirachium album serine proteinase; proteinase K; Tritirachium album proteinase K; endopeptidase K; EC 3.4.21.64; 39450-01-6 category Enzymes and Coenzymes Drugs(c1513) cas_num 39450-01-6 ECHEMI Editorial ReferencePowder beige
An enzyme that catalyzes the hydrolysis of keratin, and of other proteins with subtilisin-like specificity. It hydrolyses peptide amides. Endopeptidase K is from the mold Tritirachium album Limber. (Enzyme Nomenclature, 1992) EC 3.4.21.64.Basic Info-
Product Name:
Proteinase K
Other Name:Proteinase K;Proteinase,Tritirachium album serine;Tritirachium album proteinase K;Endopeptidase K;E.C. 3.4.21.64;Protease K;Prok;Proteinase,serine (Tritirachium album)
CAS No.:39450-01-6
InChIKeys:OUPAKHMWUJJXOM-ANOMEZDXSA-N
Molecular Weight:626.504641
Exact Mass:391.200836
EC Number:254-457-8
DSSTox ID:DTXSID6040907
HScode:35079090
Categories:
Characteristics-
PSA:
198
XLogP3:2.50
Appearance:white powder
Density:1.3±0.1 g/cm3
Refractive Index:1.648
Water Solubility:H2O: 10 mg/mL, slightly hazy, brown-yellow
Storage Conditions:2-8°C
Hazard IdentificationClassification of the substance or mixture
Skin irritation, Category 2
Eye irritation, Category 2
Respiratory sensitization, Category 1
GHS label elements, including precautionary statements
Pictogram(s) 
Signal word Danger
Hazard statement(s) H315 Causes skin irritation
H319 Causes serious eye irritation
H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled
Precautionary statement(s) Prevention P264 Wash ... thoroughly after handling.
P280 Wear protective gloves/protective clothing/eye protection/face protection/hearing protection/...
P261 Avoid breathing dust/fume/gas/mist/vapours/spray.
P284 [In case of inadequate ventilation] wear respiratory protection.
Response P302+P352 IF ON SKIN: Wash with plenty of water/...
P321 Specific treatment (see ... on this label).
P332+P317 If skin irritation occurs: Get medical help.
P362+P364 Take off contaminated clothing and wash it before reuse.
P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P304+P340 IF INHALED: Remove person to fresh air and keep comfortable for breathing.
P342+P316 If experiencing respiratory symptoms: Get emergency medical help immediately.
Storage none
Disposal P501 Dispose of contents/container to an appropriate treatment and disposal facility in accordance with applicable laws and regulations, and product characteristics at time of disposal.
Other hazards which do not result in classification
no data available
Handling and StoragePrecautions for safe handling
Handling in a well ventilated place. Wear suitable protective clothing. Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Use non-sparking tools. Prevent fire caused by electrostatic discharge steam.
Conditions for safe storage, including any incompatibilities
Store the container tightly closed in a dry, cool and well-ventilated place. Store apart from foodstuff containers or incompatible materials.
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Seller Information
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Business Type:
Manufactory
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Main Products:
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Location:
Shirley, New York 11967, USA
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Payment Terms:
TT against copy of documents,D/P
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Average lead Time:
15
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Total Annual Revenue:
$1 million-$2.5 million
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Year of Establishment:
2004
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Country Product Purchase Quantity Date Posted Post an enquiry for this product