Herbal Extract High Purity Withaferin A 5119-48-2 With Chemical Grade

Withaferin A More Info

Chemical Profile and Structural Characteristics

Withaferin A (CAS 5119-48-2) is a steroidal lactone belonging to the withanolide class of compounds, primarily isolated from Withania somnifera (Ashwagandha). Its molecular structure features an ergostane skeleton with a characteristic lactone ring at C-22 and C-26, along with multiple oxygen-containing functional groups including epoxide and hydroxyl moieties. The compound crystallizes as colorless needles with a molecular weight of 470.6 g/mol and demonstrates moderate solubility in polar organic solvents but limited water solubility. The specific stereochemistry and functional group arrangement are critical for its biological activity and molecular interactions.

Mechanism of Action and Biological Activities

The compound exerts multiple biological effects through interaction with various molecular targets. It inhibits the NF-κB signaling pathway by directly binding to and inhibiting IKKβ kinase activity, thereby suppressing inflammatory gene expression. Withaferin A also induces apoptosis through multiple mechanisms including activation of caspase cascades, generation of reactive oxygen species, and inhibition of HSP90 chaperone function. Additional activities include disruption of actin cytoskeleton dynamics through covalent binding to vimentin, anti-angiogenic effects via VEGFR2 inhibition, and modulation of autophagy processes through Beclin-1 interaction.

Therapeutic Applications and Research Significance

Research demonstrates Withaferin A's potential in oncology for various cancer types including breast, prostate, and pancreatic cancers through its pro-apoptotic and anti-proliferative activities. The compound shows significant anti-inflammatory effects in models of arthritis, inflammatory bowel disease, and neuroinflammation. Additional investigated applications include neuroprotective effects in neurodegenerative disorders, cardioprotective properties in ischemic injury models, and chemopreventive potential in carcinogenesis studies. Most research utilizes in vitro concentrations ranging from 1-10 μM and in vivo doses of 2-10 mg/kg in animal models.

Formulation Strategies and Bioavailability Enhancement

Advanced delivery systems address Withaferin A's pharmacokinetic challenges through nanoparticle formulations including liposomes, polymeric nanoparticles, and solid lipid nanoparticles that improve solubility and tissue targeting. Chemical modification approaches focus on prodrug development through esterification and glycosylation to enhance stability and absorption. We provide technical support for formulation development with compatibility data for various excipients, stability profiles under different storage conditions, and guidance on manufacturing processes to optimize bioavailability and therapeutic efficacy.

Quality Specifications and Analytical Standards

Our Withaferin A meets stringent quality specifications with HPLC purity ≥98%, comprehensive characterization by NMR and MS spectroscopy, and of related withanolides and degradation products. Critical quality parameters include heavy metals, pesticide residues, microbial limits, and residual solvents from the extraction process. We employ validated HPLC-UV methods for precise quantification and UPLC-MS for comprehensive impurity profiling. Stability studies support appropriate storage recommendations at -20°C protected from light and moisture, with demonstrated stability data for both bulk material and formulated products.

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