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Tricine CAS:5704-04-1

Name: Tricine CAS:5704-04-1
Brand: Orient
SKU: pr2511301016
Price: 4 USD
Availability: InStock

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CAS No.:

5704-04-1

Grade:

Industrial Grade

Content:

99%

Port:

SHANGHAI

Brand:

Orient

Packaging:

25kg/bag,500kg/bag, customized

Price valid (until):

2025-12-30

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Qingdao Orient Chemical Co.,LTD

Company Type:

Trader

Location:

China

Qualification:

Main Products:

pharmaceutical intermediates,peptides,electronic chemicals

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Product Description
Seller Information

Description

Tricine Basic information

Descriptioin Reference

Product Name:	Tricine
Synonyms:	LABOTEST-BB LT00454966;n-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-glycin;TRICINE;TRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDE;TRIMETHYLOLAMINOMETHANE HYDROCHLORIDE;TRIS-HCL BUFFER;TRIClNE;E-Z 96 SIZE SELECT-IT (1 X 96)
CAS:	5704-04-1
MF:	C6H13NO5
MW:	179.17
EINECS:	227-193-6
Product Categories:	Biochemistry;Good's Buffers;Buffer
Mol File:	5704-04-1.mol

Tricine Chemical Properties

Melting point	186-188 °C (dec.)
Boiling point	311.69°C (rough estimate)
bulk density	500kg/m3
density	1.05 g/mL at 20 °C
vapor pressure	0.002-0.004Pa at 20-25℃
refractive index	1.4240 (estimate)
storage temp.	Keep in dark place,Inert atmosphere,Room temperature
solubility	H₂O: 4 M at 20 °C, clear, colorless
form	crystalline
color	clear colorless (40 % (w/w) in H₂O) solution
Odor	Odorless
PH	4.0-6.0 (1M in H2O)
PH Range	7.4 - 8.8
pka	8.1(at 25℃)
biological source	synthetic
Water Solubility	soluble
Merck	14,9651
BRN	1937804
Stability:	Stable.
LogP	-2.87 at 25℃
CAS DataBase Reference	5704-04-1(CAS DataBase Reference)
EPA Substance Registry System	Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]- (5704-04-1)
Absorption	≤0.05 at 280 in H2O at 1M ≤0.1 at 260 in H2O at 1M

Safety Information

Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-36-36/37/39
WGK Germany	3
F	3
TSCA	Yes
HS Code	29052900

Tricine Usage And Synthesis

Descriptioin	Tricine is a zwitterionic amino acid. It is a white crystalline powder. It was first prepared by Good to buffer chloroplast reaction.1 Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets^.1,2 Attributing to its higher negative charge than glycine, it can migrate faster. At the same time, its high ionic strength results in more ion movement and less protein movement. Therefore, it works as a buffer component for separation of low molecular weight peptides.³
Reference	S. R. Haider, B. L. Sharp, H. J. Reid, A comparison of Tris‐glycine and Tris‐tricine buffers for the electrophoretic separation of major serum proteins, Journal of Separation Science, 2011, vol. 34, pp. 2463-2467 H. Schägger, G. von Jagow, Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Analytical Biochemistry, 1987, vol. 166, pp. 368-379
Chemical Properties	White/clear crystalline powder
Uses	Good's buffers Tricine is used as an electrophoresis buffer and involved in the separation of low molecular weight proteins and peptides. It operates in the pH range between 7.4 and 8.8. It acts as an effective scavenger of hydroxyl radicals during the study of radiation-induced membrane damage. It is also used in resuspension of cell pellets. It is a zwitterionic amino acid commonly useful in the pH range of 7.4-8.8 and the pKa is 8.05 at 25°C. This buffer is also used as an eluent in asymmetrical flow field-flow fractionation (AFFFF).
Definition	ChEBI: N-tris(hydroxymethyl)methylglycine is a Good's buffer substance, pKa = 8.15 at 20 ℃. It is functionally related to a member of tris and a glycine. It is a tautomer of a N-tris(hydroxymethyl)methylammonioacetate.
General Description	Tricine and its salts are used as biological buffers to control the pH of body fluids in vitro and in vivo. It acts as a precursor for polymers, oxazolones (with carboxylic acids), and oxazolidines (with aldehydes). Trizma can serve as an alkalizing agent in treating acidosis of the blood.
Biological Activity	Electrophoresis buffer. Working pH range: 7.4 - 8.8. Commonly used in SDS-PAGE to seperate peptides. Also used in other electrophoresis methods, high performance liquid chromatography and ion exchange chromatopgraphy.
Purification Methods	Crystallise Tricine from EtOH and water. [Good et al. Methods Enzymol 24B 53 1968, McGothlin & Jordan Analyt Lett 9 245 1976, Beilstein 18 III/IV 3454.]

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—— Q&A ——

Q1:Do you provide sample?

A: Yes, we can provide free sample, you only need pay the shipping cost.

Q2: What is the lead time?

A: For stock goods, it usually take about 5-7 days by air, 15-30 days by sea,For customized products, the delivery time needs to be confirmed based on the actual situation.

Q3: How can I buy? What is the payment method?

A: You can contract us through Email or you can browse our website:orientchem.cc, we have bank transfer,

A：We attach great importance to after-sales service of our products. If there are any quality issues, please contact us within 30 days of receiving the goods and we will handle returns and exchanges for you.

Certificates

Basic Info

Product Name:

Tricine

Other Name:

Glycine,N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-;N-[2-Hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine;Tricine;N-[Tris(hydroxymethyl)methyl]glycine;NSC 369995;2-[[1,3-Dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid;1644287-59-1

CAS No.:

5704-04-1

Molecular Formula:

C6H13NO5

InChIKeys:

InChIKey=SEQKRHFRPICQDD-UHFFFAOYSA-N

Molecular Weight:

179.17100

Exact Mass:

179.17

EC Number:

227-193-6

UNII:

W12LH4V8V3

NSC Number:

369995

DSSTox ID:

DTXSID1063982

HScode:

2922509090

Categories:

Bulk Drug Intermediates

Characteristics

PSA:

110.02000

XLogP3:

-5.1

Appearance:

white crystalline powder

Density:

1.05g/mLat20ºC

Melting Point:

187 °C

Boiling Point:

500.9ºC at 760 mmHg

Flash Point:

256.7ºC

Refractive Index:

1.553

Water Solubility:

H2O: soluble

Storage Conditions:

Store at RT.

See More Tricine Properties 

Hazard Identification

Classification of the substance or mixture

Not classified.

GHS label elements, including precautionary statements

Precautionary statement(s)
Pictogram(s)	No symbol.
Signal word	No signal word
Hazard statement(s)	none
Prevention	none
Response	none
Storage	none
Disposal	none

Other hazards which do not result in classification

no data available

Handling and Storage

Precautions for safe handling

Handling in a well ventilated place. Wear suitable protective clothing. Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Use non-sparking tools. Prevent fire caused by electrostatic discharge steam.

Conditions for safe storage, including any incompatibilities

Store the container tightly closed in a dry, cool and well-ventilated place. Store apart from foodstuff containers or incompatible materials.

See More Tricine Safety Data Sheet 

Seller Information

Business Type:
Trader

Main Products:
pharmaceutical intermediates,peptides,electronic chemicals

Location:
Room 102, 1st Floor, East Office Building, No. 45 Pengwan Road, Qianwan Bonded Port Area, Qingdao Zone, China (Shandong) Pilot Free Trade Zone

Payment Terms:
100% TT in advance

Average lead Time:
10

Total Annual Revenue:
$10 million-$25 million

Total Employees:
51-100

Year of Establishment:
2024

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We are mainly specilized in supply of pharmaceutical intermediates, electronic chemicals，food additives, and other fine chemical products.
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Website: orientchem.cc
Email: scarlett@orientchem.cc
WhatsApp: +86 17753254221
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