Dihydrokaempferol-7-O-rhamnoside 97% Purity Flavonoid Glycoside Reference Standard for Natural Product Analysis and Antioxidant Research CAS 69135-41-

Product Overview

Dihydrokaempferol-7-O-rhamnoside (CAS 69135-41-7) is a high‑purity flavonoid glycoside reference standard featuring dihydrokaempferol (3,4',5,7‑tetrahydroxyflavanone) as the aglycone with a rhamnose moiety attached at the 7‑position. The molecular formula is C₂₁H₂₂O₁₀ with a molecular weight of 434.39 g/mol. Dihydrokaempferol, also known as aromadendrin, is a flavanone that serves as a key intermediate in the biosynthesis of flavonols and other flavonoids. This compound is naturally found in various plant species and has been studied for its antioxidant, anti‑inflammatory, and hepatoprotective properties. As a reference standard, it is valuable for the identification and quantification of dihydroflavonol glycosides in plant extracts and herbal products using HPLC‑DAD and UPLC‑MS/MS methods.

Synthetic Process / Isolation Method

This compound is typically isolated from natural plant sources through a series of extraction and chromatographic purification steps:

• Extraction: Dried plant materials (such as leaves or bark of various tree species) are extracted with aqueous ethanol or methanol.

• Partitioning: The crude extract is subjected to liquid‑liquid partitioning with ethyl acetate or n‑butanol to concentrate the flavonoid fraction.

• Column Chromatography: The enriched fraction undergoes repeated column chromatography using silica gel, polyamide, or Sephadex LH‑20, eluting with gradient solvent systems such as chloroform‑methanol‑water or ethyl acetate‑methanol‑water.

• Preparative HPLC: Final purification is achieved using preparative HPLC with a C18 reversed‑phase column.

• Recrystallization: The purified compound is recrystallized to achieve ≥97% purity.

• Quality Confirmation: The final product is rigorously characterized by HPLC‑PDA, ¹H‑NMR, ¹³C‑NMR, and high‑resolution mass spectrometry.

Mechanism of Action

As a dihydroflavonol glycoside, dihydrokaempferol-7-O-rhamnoside exerts its biological activities through multiple mechanisms:

• Antioxidant Activity: The phenolic hydroxyl groups effectively scavenge free radicals and chelate metal ions, protecting cells from oxidative stress.

• Anti‑inflammatory Activity: Modulates inflammatory signaling pathways, reducing the production of pro‑inflammatory cytokines.

• Hepatoprotective Effects: Protects liver cells from oxidative damage and may have therapeutic potential in liver diseases.

• Enzyme Inhibition: May inhibit certain enzymes involved in inflammatory and oxidative stress pathways.

Quality Specifications

Our Dihydrokaempferol-7-O-rhamnoside is manufactured under strict quality control conditions: - Purity: ≥97.0% determined by HPLC - Identity confirmed by NMR and mass spectrometry - Water content: ≤1.0% - Heavy metals: ≤20 ppm - Each batch accompanied by comprehensive Certificate of Analysis (COA) - Stable for 24 months when stored at 2‑8°C in sealed containers protected from light and moisture

Packaging & Shipping

We offer flexible packaging options to meet diverse customer needs: - Research quantities: 2mg, 5mg, 10mg amber glass vials with PTFE‑lined caps, purged with inert gas - Bulk quantities: Available upon request - Custom packaging available upon request - Shipped at ambient temperature for routine orders - Cold chain shipping available upon request for temperature‑sensitive orders - International shipping available with proper documentation

Jinan Topfull Chemical Co., Ltd. is an international company rooted in China, specializing in the export of chemical products.

Our company is committed to making every effort to provide ideal chemical products to potential customers worldwide, including but not limited to: organic/inorganic reagents, biological reagents, plant extracts, pharmaceutical intermediates, active pharmaceutical ingredients (APIs), reference standards, reference substances, peptides, and more. Meanwhile, we have established long-term and stable cooperative relationships with university laboratories and off-campus research institutions, enabling us to offer tailored product services based on customers' actual needs.

We currently support customized services for most product categories, with supply quantities ranging from milligram (mg), gram (g) to kilogram (kg) levels, as well as wholesale. Product purity covers the full spectrum from 90% to 99.9%, providing customers with one-stop convenient services.

To date, our products have been exported to developed countries such as the United States, Australia, Canada, Mexico, as well as EU member states including France, Germany, and many other countries and regions such as Nigeria, Ghana, Southeast Asia, and other parts of Asia. Our product quality and services have consistently received high praise.

Looking ahead, our company will continue to serve as a bridge connecting China and the world, delivering high-quality products and services to global customers.