Bromelain

Press to wash the pineapple skin, squeeze out the juice through a juicer, add 0.5g of sodium benzoate (ie 0.05%) per liter of skin juice, as a preservative, send it to the raw material warehouse for low-temperature storage for later use. Sodium benzoate → squeezed juice adsorption and elution Put the juice in a stainless steel vacuum flask, and add 40g (4%) of kaolin per liter of juice under constant stirring and rotating speed of 40-60r/min. At a temperature of 100C, adsorb for 15 to 20 minutes, and let it stand still. On the next day, when the white clay settles well, siphon, remove the supernatant, leave the wet clay, add saturated sodium carbonate or sodium oxyfluoride (66g/L or 6.6%) to adjust the pH to 6.5～7, and then add the wet soil. 50% ammonium sulfate or edible refined salt, stir for 25-30 min, and eluate to obtain the eluate. The eluate is filtered with a filter press to obtain the filtrate. Juice white clay; 100→adsorbed substance Na2CO3; (NH4)^2SO4→^elution; press filter press filtrate salting out, suction filter press filtrate into a steel drum, adjust the pH to 4.5～5 with 9% hydrochloric acid, Under gentle agitation with a wooden stick, add 200～250g (20%～25%) ammonium sulfate (or 250g refined salt) per liter of this filtrate for salting out to dissolve the ammonium sulfate (refined salt) and put it in the cold storage. The next day, the upper ammonium sulfate solution was siphoned out, the dilute enzyme paste was obtained by the lower chromatography, and the crude enzyme paste was obtained by centrifugal separation. Suction filtration through a Buchner funnel to remove part of the ammonium sulfate solution. Store under -20%<0, to be refined. Eluent HCl; ((NH4)^2^)SO4→salting out; Suction and filter the crude product to dissolve and precipitate. Take the crude enzyme and add 8 to 10 times the amount of tap water, and then add 160g (16 %) Sodium hydroxide adjusts the pH from 7 to 75, and gently stirs until dissolved. In order to prevent foaming, press and filter quickly to separate and remove impurities to obtain a clear liquid. The pipeline and filter press are rinsed with a small amount of tap water, and the washing liquid and the clear liquid are combined. Under constant stirring, adjust the pH to 4 with 9% hydrochloric acid, wait for the enzyme to precipitate, and centrifuge to obtain the wet bromelain. Tap water for crude products; pH7～7.5→dissolved clarified liquid HCl;pH4→precipitate refined products drying and crushing. Suction filtration of wet enzyme products, causing cracks in the filter cake. The fan is half-dried to remove part of the water, dried in vacuum, and then crushed by a ball mill , That is, the finished bromelain. Calculated according to the quality of pineapple, the yield is 0.3725g/ton, and the content is 300, 000 HUF units/g. Refined product drying; crushing→finished product

Bromelain is commonly used in bronchitis, bronchial asthma, acute pneumonia, postpartum breast hemorrhage, mastitis, postpartum thrombosis, retinitis, etc. Inflammation, edema and hematoma caused by various causes and thrombosis. Combined with antibacterial drugs to treat arthritis, periarthritis, cellulitis, calf ulcers, various inflammations of the respiratory system and urinary tract infections. But after taking the drug, there are gastrointestinal reactions such as nausea, vomiting, diarrhea, loss of appetite, loose stools, and digestive tract ulcers. Occasional nosebleeds, menorrhagia, dysmenorrhea and uterine bleeding. The tablets should be swallowed and not chewed to avoid being destroyed by pepsin and ineffective. Severe liver and kidney diseases, those who are using anticoagulants or blood coagulation bacteria can not be used. Oral, 3 squeeze to wash the pineapple skin, squeeze out the juice through the juicer, add 0.5g of sodium benzoate (ie 0.05%) per liter of skin juice, as a preservative, send it to the raw material store for low temperature storage. Sodium benzoate→Squeeze juice absorption, elution Place the juice in a stainless steel thermos flask, add 40g (ie 4%) of white clay per liter of juice under constant stirring at a speed of 40-60r/min, At a temperature of 100C, adsorb for 15 to 20 minutes and let stand for approval. The next day until the white clay settles well, siphon, remove the supernatant, and leave the wet clay, add sodium carbonate saturated solution or sodium oxyfluoride (66g/L or 6.6%) to adjust the pH to 6.5-7, and then add the wet clay 50% ammonium sulfate or edible refined salt, stirring for 25-30min, elution, to obtain eluent, the eluent is filtered with a filter press to obtain filtrate. Juice white clay; 100→adsorbed adsorbate Na2CO3; (NH4)^2SO4→^ elution; salting out of the pressure filtration pressure filtrate, suction filtration pressure filtrate is placed in a steel drum, adjusted to pH4.5~5 with 9% hydrochloric acid, With light agitation with a wooden stick, 200-250g (20%-25%) of ammonium sulfate (or 250g of refined salt) is added to each liter of this filtrate for salting out, so that the ammonium sulfate (refined salt) is dissolved and placed in the cold storage. The next day, the upper ammonium sulfate solution was siphoned out, and the diluted enzyme paste was chromatographed under the lower chromatography, and centrifuged to obtain the crude enzyme paste. Suction filtration through a Buchner funnel to remove part of the ammonium sulfate solution. Store at -20%<0, to be refined. Eluent HCl; ((NH4)^2^)SO4→salting out; dissolve and precipitate the crude product by suction filtration. Take 8-10 times the amount of tap water for the crude enzyme, and then add 160g (16 %) Sodium hydroxide adjusts the pH to 7 to 75 and gently stirs to dissolve. In order to prevent foaming, press filter quickly to separate and remove impurities to get a clear liquid. The pipeline and filter press are rinsed with a small amount of tap water, and the washing liquid and the clarified liquid are combined. Under constant stirring, adjust the pH4 with 9% hydrochloric acid. After the enzyme is precipitated, centrifuge to obtain wet bromelain. Crude product tap water; pH7～7.5→dissolve clarified solution HCl; pH4→precipitation refined product drying, crushing Wet enzyme products are filtered by suction, causing cracks in the filter cake, the fan is semi-dry, remove part of the water, vacuum drying, and then crushed by ball mill , You get the finished bromelain. Based on the quality of pineapple, the yield is 0.3725g/ton and the content is 300,000 forint units/g. Oral, 30,000 to 100,000 units/time, 3-4 times/day. External application of 0.1% to 0.2% physiological saline solution is applied to wounds with chronic necrosis and chronic ulcers. Pineapple enzyme enteric-coated tablets. Oral 1 to 3 tablets, 3 to 4 times a day, compound pineapple enzyme tablets; Oral 2 to 4 tablets, 3 times a day.