Acridine Orange
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Acridine Orange
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CAS No:
65-61-2
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Formula:
C17H19N3.ClH
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Chemical Name:
Acridine Orange
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Synonyms:
3,6-Acridinediamine,N3,N3,N6,N6-tetramethyl-,hydrochloride (1:1);Acridine,3,6-bis(dimethylamino)-,monohydrochloride;3,6-Acridinediamine,N,N,N′,N′-tetramethyl-,monohydrochloride;Acridine Orange R;Acridine,3,6-bis(dimethylamino)-,hydrochloride;C.I. 46005;Basic Orange 3RN;Acridine Orange NO;C.I. Basic Orange 14;Acridine Orange;Rhoduline Orange NO;3,6-Bis(dimethylamino)acridine hydrochloride;Acridine Orange N;Basic Orange 14;Acridine Orange NS;Sumitomo Acridine Orange NO;Sumitomo Acridine Orange RK conc
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CAS No:
Description
Acridine orange is a cell-permeable fluorescent dye that binds to nucleic acids, resulting in an altered spectral emission.
A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.
Characteristics
19.37000
4.32200
orange to red Powder
1.001 g/mL at 20ºC
284-287 °C(lit.)
468.6ºC at 760 mmHg
237.2ºC
n20/D 1.338
Soluble in water, ethanol, dimethyl sulfoxide
-20°C
dnd-mus:ast 20 mmol/L BBACAQ 374,96,74
Thermal decomposition of toxic chloride, hydrogen chloride and nitrogen oxide fumes
Safety Information
NONH for all modes of transport
3
22-24/25
AR7601000
Ventilated, low temperature and dry
P201, P202, P281, P308+P313, P405, P501
H340
|Danger|H340 (99.15%): May cause genetic defects [Danger Germ cell mutagenicity]|P201, P202, P281, P308+P313, P405, and P501|Aggregated GHS information provided by 117 companies from 2 notifications to the ECHA C&L Inventory.
Drug Information
Chemical agents that increase the rate of genetic mutation by interfering with the function of nucleic acids. A clastogen is a specific mutagen that causes breaks in chromosomes. (See all compounds classified as Mutagens.)|Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags. (See all compounds classified as Fluorescent Dyes.)
3,6-Bis(dimethylamino)acridine
Acridine Orange Use and Manufacturing
Acridine orange is a dye that interacts with DNA and RNA by intercelation or electrostatic attraction. This cationic dye has been used as a fluorescent stain for nucleic acids in agarose and polyacrylamide gels.
3,6-Acridinediamine, N3,N3,N6,N6-tetramethyl-, hydrochloride (1:1): ACTIVE
Computed Properties
Molecular Weight:301.8
Hydrogen Bond Donor Count:1
Hydrogen Bond Acceptor Count:3
Rotatable Bond Count:2
Exact Mass:301.1345753
Monoisotopic Mass:301.1345753
Topological Polar Surface Area:19.4
Heavy Atom Count:21
Complexity:298
Covalently-Bonded Unit Count:2
Compound Is Canonicalized:Yes
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