Acid Red 94

bordeaux-red to red-brown crystalline powder

Rose bengal is a xanthene dye, fluorescein derivative, and photosensitizer. It exhibits absorption/emission maxima of 548/567 nm, respectively. Rose bengal binds toS. aureuscells and decreases survival of photoirradiatedS. aureusto 0.012% when used at a concentration of 1 μM. It generates singlet oxygen when exposed to photoirradiation in cell-free assays and induces potassium ion leakage fromS. aureusand bovine erythrocytes in the presence of photoirradiation. Rose bengal inhibits the cytochrome P450 (CYP) isoform CYP3A4/5 and the UDP-glucuronosyltransferase (UGT) isoform UGT1A6 in human liver microsomes in a light-dependent manner, with IC50values of 0.072 and 0.035 μM, respectively, in yellow light, 3.1 and 4.2 μM, respectively, in ambient light, and 3 and 4.2 μM, respectively, in the dark. It has been used for staining of live cells, but exhibits both intrinsic and phototoxicity.

This ELISA antibody pair detects Bovine IGF-1

An experimental teratogen. Experimental reproductive effects. Questionable carcinogen with experimental neoplastigenic data. When heated to decomposition it emits toxic fumes of Cl-, I-, and NazO.

This biological stain can be purified by chromatography on silica TLC using a 35:65 mix of EtOH/acetone as eluent. [Beilstein 19 II 261, 19 III/IV 2926.]

Method 1. The process of recombinant TNFα bacterial fermentation. The engineered bacteria were inoculated into LB medium, cultured at 37°C with shaking to A600nm=1, transferred to M9 medium, and induced at 42°C for 4h. Centrifuge to collect bacteria. The cells were suspended in phosphate buffer (PB, 10mmol/L) with a pH of 8, and the cells were disrupted by ultrasound, the supernatant was collected by centrifugation, and the PB was dialyzed to obtain crude TNFα. Engineering bacteria [culture, fermentation]→[37℃, 42℃] fermentation cell [10mmol/L PB]→[pH8] TNFα crude DEAE-Sepharose FF ion exchange column chromatography ion exchange column equilibrated with PB, plus TNFα crude, Wash the column with PB, then add 75 mmol/L NaCl for elution, collect each peak, and measure TNFα activity. Crude TNFα [DEAE-Sepharose FF ion column]→[75 mmol/L NaCl] TNFα active component IQ-Sepharose FF ion exchange column chromatography ion exchange column equilibrated with pH 8, 20 mmol/LTris-HCl buffer, and then added The TNFα active component I was eluted with 80 mmol/L NaCl, the peaks were collected, and the TNFα activity was measured to obtain the TNFα active component II. TNFα active component I [Q-Sepharose FF ion column]→[80 mmol/L NaCl] TNFα active component IICM-Sepharose FF ion exchange column chromatography ion exchange column equilibrated with 10mmol/L PB at pH 6, plus TNFα The active component II is eluted step by step with the PB solution, PB solution plus 200 mmol/L NaCl and PB solution plus 600 mmol/L NaCl. Collect each eluate and measure the TNFα activity to obtain the pure TNFα product (in At 4℃, through three normal pressure ion exchange, electrophoresis showed that the elution peak of PB solution plus 600 mmol/L NaCl is a single TNFα band, and pure TNFα can be obtained). TNFα active component II [CM-Sepharose FF ion column]→[PB, 200 and 600mmol/L NaCl] TNFα pure product Method two, the synthetic TNFα mutation modification mutation modification using multi-site mutation primers and PCR method to synthesize The TNFα gene is modified by mutation, so that the second arginine codon CGT becomes lysine codon AAA, and the mutant gene TNF-K2 is obtained. TNFα gene [multi-site mutation primer, PCR method]→TNF-K2 transformation and recombination Insert TNF-K2 into the downstream of the PL promoter of the vector pSB-92, and transform E. coliJM103, the transformants were identified by restriction enzyme digestion, and the recombinant plasmid pSB-TNF-K2 was obtained. TNF-K2[pSB-92]→pSB-TNF-K2 culture and induction The pSB-TNF-K2 was cultured at 30℃ to logarithmic growth phase, and induced at 42℃ for 4h, then the mutant [Lys2]TNFα, its molecular weight It is 17kD and the specific activity is 6.8×107U/mg protein. It is a soluble protein. pSB-TNF-K2[30℃, 42℃, 4h]→[Lys2]TNFα.

Bacterial stain; adsorption indicator for silver titration

For research use only. Not for use in diagnostic procedures.Please refer to the attachedProtocolfor details.

Rose Bengal Sodium Salt is used in biological studies as potential for use as sensitizers in photodynamic and sonodynamic therapy by synthesizing Rose Bengal amphiphilic derivatives. This compound has neuroprotective properties.

Molecular Weight:1017.6
Hydrogen Bond Acceptor Count:5
Exact Mass:1017.4601
Monoisotopic Mass:1015.4631
Topological Polar Surface Area:81.6
Heavy Atom Count:35
Complexity:780
Covalently-Bonded Unit Count:3
Compound Is Canonicalized:Yes