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Rose Bengal sodium salt

Rose Bengal sodium salt structure

CAS No:
632-69-9
Formula:
C20H4Cl4I4O5.2Na
Chemical Name:
Rose Bengal sodium salt
Synonyms:
Spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one,4,5,6,7-tetrachloro-3′,6′-dihydroxy-2′,4′,5′,7′-tetraiodo-,sodium salt (1:2);Fluorescein,4,5,6,7-tetrachloro-2′,4′,5′,7′-tetraiodo-,disodium salt;Spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one,4,5,6,7-tetrachloro-3′,6′-dihydroxy-2′,4′,5′,7′-tetraiodo-,disodium salt;3′,4′,5′,6′-Tetrachloro-2,4,5,7-tetraiodofluorescein disodium salt;Sodium tetraiodotetrachlorofluorescein;Rose Bengal sodium;Rose Bengal Extra;Rose Bengal disodium salt;Japan Red 105-1;2′,4′,5′,7′-Tetraiodo-3,4,5,6-tetrachlorofluorescein disodium salt;Red No. 105-1;Rose Bengal disodium;Rose Bengal sodium salt;Acid Red 94 disodium salt;PV 10;15816-66-7;18265-56-0;51799-27-0;55711-73-4;74198-03-1;157367-41-4;192930-93-1;198831-91-3;337360-08-4;410097-66-4;677032-84-7;861686-24-0;951248-21-8;1245731-28-5;1350479-68-3;1622253-48-8;2438210-61-6
Categories:
Dyes and Pigments > Dye

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Description

bordeaux-red to red-brown crystalline powder

Rose Bengal sodium salt Basic Attributes

Molecular Weight：

1017.64

Exact Mass：

1015.463013

BRN：

3645857

EC Number：

211-182-8

HScode：

32129000

Characteristics

PSA：

93.40000

XLogP3：

8.10410

Appearance：

Red-brown Solid

Melting Point：

>300°C

Boiling Point：

757.8ºC at 760 mmHg

Flash Point：

412.1ºC

Water Solubility：

H2O: soluble 1mg/mL

Storage Conditions：

Store at +15°C to +30°C.

Safety Information

Transport codes：

NONH for all modes of transport

wgk germany：

hazard class code：

36-36/38

safety instructions：

22-24/25-37/39-26

RTECS Number：

LM5920000

Marks：

Precautionary statement(s)：

P261, P264, P271, P280, P302+P352, P304+P340, P305+P351+P338, P312, P321, P332+P313, P337+P313, P362, P403+P233, P405, P501

Hazard Statements：

H315

Rose Bengal sodium salt Use and Manufacturing

Methods of Manufacturing

Method 1. The process of recombinant TNFα bacterial fermentation. The engineered bacteria were inoculated into LB medium, cultured at 37°C with shaking to A600nm=1, transferred to M9 medium, and induced at 42°C for 4h. Centrifuge to collect bacteria. The cells were suspended in phosphate buffer (PB, 10mmol/L) with a pH of 8, and the cells were disrupted by ultrasound, the supernatant was collected by centrifugation, and the PB was dialyzed to obtain crude TNFα. Engineering bacteria [culture, fermentation]→[37℃, 42℃] fermentation cell [10mmol/L PB]→[pH8] TNFα crude DEAE-Sepharose FF ion exchange column chromatography ion exchange column equilibrated with PB, plus TNFα crude, Wash the column with PB, then add 75 mmol/L NaCl for elution, collect each peak, and measure TNFα activity. Crude TNFα [DEAE-Sepharose FF ion column]→[75 mmol/L NaCl] TNFα active component IQ-Sepharose FF ion exchange column chromatography ion exchange column equilibrated with pH 8, 20 mmol/LTris-HCl buffer, and then added The TNFα active component I was eluted with 80 mmol/L NaCl, the peaks were collected, and the TNFα activity was measured to obtain the TNFα active component II. TNFα active component I [Q-Sepharose FF ion column]→[80 mmol/L NaCl] TNFα active component IICM-Sepharose FF ion exchange column chromatography ion exchange column equilibrated with 10mmol/L PB at pH 6, plus TNFα The active component II is eluted step by step with the PB solution, PB solution plus 200 mmol/L NaCl and PB solution plus 600 mmol/L NaCl. Collect each eluate and measure the TNFα activity to obtain the pure TNFα product (in At 4℃, through three normal pressure ion exchange, electrophoresis showed that the elution peak of PB solution plus 600 mmol/L NaCl is a single TNFα band, and pure TNFα can be obtained). TNFα active component II [CM-Sepharose FF ion column]→[PB, 200 and 600mmol/L NaCl] TNFα pure product Method two, the synthetic TNFα mutation modification mutation modification using multi-site mutation primers and PCR method to synthesize The TNFα gene is modified by mutation, so that the second arginine codon CGT becomes lysine codon AAA, and the mutant gene TNF-K2 is obtained. TNFα gene [multi-site mutation primer, PCR method]→TNF-K2 transformation and recombination Insert TNF-K2 into the downstream of the PL promoter of the vector pSB-92, and transform E. coliJM103, the transformants were identified by restriction enzyme digestion, and the recombinant plasmid pSB-TNF-K2 was obtained. TNF-K2[pSB-92]→pSB-TNF-K2 culture and induction The pSB-TNF-K2 was cultured at 30℃ to logarithmic growth phase, and induced at 42℃ for 4h, then the mutant [Lys2]TNFα, its molecular weight It is 17kD and the specific activity is 6.8×107U/mg protein. It is a soluble protein. pSB-TNF-K2[30℃, 42℃, 4h]→[Lys2]TNFα.

Uses

Bacterial stain; adsorption indicator for silver titration

Manufacturing Info：

Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 4,5,6,7-tetrachloro-3',6'-dihydroxy-2',4',5',7'-tetraiodo-, sodium salt (1:2): ACTIVE

Computed Properties

Molecular Weight:1017.6
Hydrogen Bond Acceptor Count:5
Exact Mass:1017.4601
Monoisotopic Mass:1015.4631
Topological Polar Surface Area:81.6
Heavy Atom Count:35
Complexity:780
Covalently-Bonded Unit Count:3
Compound Is Canonicalized:Yes

Material

Sodium chloride

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