MOPS

CBI

DryPowder

3-(N-morpholino)propanesulfonic acid is a Good's buffer substance, pKa = 7.2 at 20 ℃. It is a member of morpholines, a MOPS and an organosulfonic acid. It is a conjugate acid of a 3-(N-morpholino)propanesulfonate. It is a tautomer of a 3-(N-morpholiniumyl)propanesulfonate.

wherein the biological buffer is selected from the group consisting of: ... N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES); N-2-hydroxyethylpiperazine-N-2-propanesulfcnic acid (HEPPSO); 2-N-morpholinoethanesulfonic acid (MES); 4-N-morpholinobutanesulfonic acid (MOBS); 3-N-morpholinopropanesulfonic acid (MOPS); 3-N-morpholino-2-hydroxypropanesulfonic acid (MOPSO); piperazine-N-N-bis-2-ethanesulfonic acid (PIPES); piperazine-N-N-bis-2-hydroxypropanesulfonic acid (POPSO); ...wherein the biological buffer is selected from the group consisting of: ... N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES); N-2-hydroxyethylpiperazine-N-2-propanesulfonic acid (HEPPSO); 2-N-morpholinoethanesulfonic acid (MES); 4-N-morpholinobutanesulfonic acid (MOBS); 3-N-morpholinopropanesulfonic acid (MOPS); 3-N-morpholino-2-hydroxypropanesulfonic acid (MOPSO); piperazine-N-N-bis-2-ethanesulfonic acid (PIPES); piperazine-N-N-bis-2-hydroxypropanesulfonic acid (POPSO); ..., wherein the plurality of positively charged groups are provided by a biological buffer which is selected from the group consisting of: ... -2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES); -2-hydroxyethylpiperazine-N-2-propanesulfonic acid (HEPPSO); 2-N-morpholinoethanesulfonic acid (MES); 4-N--morpholinobutanesulfonic acid (MOBS); 3-N-morpholinopropanesulfonic acid (MOPS); 3-N-morpholino-2-hydroxypropanesulfonic acid (MOPSO); piperazine-N-N-bis-2-ethanesulfonic acid (PIPES); piperazine-N-N-bis-2-hydroxypropanesulfonic acid (POPSO); ......econd, higher, pH at which the charge on the ionizable groups is negative, neutral or less positive, wherein said plurality of positively ionizable groups have a pKa between about 4.5 and about 8.5, the ionizable groups being provided by a biological buffer selected from the group consisting of: ... -2-hydroxyethylpiperazine-N-2-ethanesulfonic acid (HEPES); -2-hydroxyethylpiperazine-N-2-propanesulfonic acid (HEPPSO); 2-N-morpholinoethanesulfonic acid (MES); 4-N-morpholinobutanesulfonic acid (MOBS); 3-N-morpholinopropanesulfonic acid (MOPS); 3-N-morpholino-2-hydroxypropanesulfonic acid (MOPSO); piperazine-N-N-bis-2-ethanesulfonic acid (PIPES); piperazine-N-N-bis-2-hydroxypropanesulfonic acid (POPSO); ...A. 3-(N-Morpholino) Propane Sulfonic Acid (Hereafter Abbreviated as MOPS) Buffer Solution: 2 mM CaCl2 was added to 20 mM MOPS (pH 7).Examples of the Good's buffer include, but are not limited to, ... N-(2-acetamide) iminodiacetic acid (ADA), piperazine-N, N'-bis(2-ethanesulfonic acid) (PIPES), N-(2-acetamide)-2-aminoethanesulfonic acid (ACES), 3-morpholino-2-hydroxypropanesulfonic acid (MOPSO), 3-morpholinopropanesulfonic acid (MOPS), N-[tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid (TES), 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES), N-[tris(hydroxymethyl)methyl]glycine (Tricine), ...Preparation of [C1ted][MOPS]; [C1ted][MOPS] is an ionic liquid comprised of the cation, 1-methyl-1, 4-diazabicyclo[2.2.2]octanium ([C1ted]), and as the anion, 3-morpholinopropanesulfonate ([MOPS]).A 21.859-g flask was loaded sequentially with MOPS (1.247 g, 5.96 mmol) and aqueous [C1ted][OH] (0.92 M, 6.480 mL, 5.96 mmol). Water was removed by rotary evaporation to produce [C1ted][MOPS] (1.996 g, 100%, nominally -0.15 wt % water) as a viscous, colorless ionic liquid. The ionic liquid of was liquid at ambient conditions and had an electrochemical window of 7V with a platinum working electrode.General procedure: 2500L of pretreated anhydrous ethanol (water content <0.02%) was added to a 5000L glass-lined reactor, and 800 kg of molten 1, 3-PS was added under stirring. The mixture was stirred and cooled to 25-30 C, and the temperature was controlled for 4 hours.582 kg of morpholine was added dropwise, and the reaction was carried out at this temperature for 2 hours, and dropped to 0 C.The crystal was incubated for 10 hours, and 2160 kg of wet product was obtained by centrifugation, and the alcohol obtained by the mother liquor was recovered for recrystallization. Add 1600L of alcohol to the 3000L glass jar, add 300kg of deionized water, add 1080kg of the wet product obtained by the step, stir to 70 C, add deionized water to make the wet product completely soluble, add 1kg of insurance powder, 25kg of medicine Using activated carbon, decolorize for 1 hour, press filter, the filtrate enters the crystallization tank, and then add 1600L alcohol to wash the tank. Pass the filter to enter the crystallization tank. Under nitrogen protection, heat up and dissolve. After total dissolution, slowly stir and cool down to 35~40 C. When it becomes turbid, it will be moisturized and crystallized for 1 hour, then cooled to 0~5 C, centrifuged, washed twice with 20L of absolute ethanol, dried, placed in a rotary vacuum dryer, controlled temperature below 80 C, vacuum degree above 0.08MPa , dry to the product qualified, get 548kg of product, yield 80% (in terms of 1, 3-PS), sampling test: content 99.8% (alkali titration), The UV absorbance of the 0.5 M aqueous solution at 260 nm and 280 nm was 0.02.

MOPS (3-(N-morpholino)propanesulfonic acid) is a buffer introduced by Good et al. in the 1960s. It is a structural analog to MES. Its chemical structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.

Intermediates

Non-TSCA use

Molecular Weight:209.27
XLogP3:-3.1
Hydrogen Bond Donor Count:1
Hydrogen Bond Acceptor Count:5
Rotatable Bond Count:4
Exact Mass:209.07217913
Monoisotopic Mass:209.07217913
Topological Polar Surface Area:75.2
Heavy Atom Count:13
Complexity:227
Covalently-Bonded Unit Count:1
Compound Is Canonicalized:Yes