Intraperitoneal injection of the drug into mice with cholestatic jaundice induced by 1-naphthyl isothiocyanate can reduce serum aspartate aminotransferase, alanine aminotransferase and serum conjugated bilirubin levels, and alleviate liver cell damage in the model animals.

It works at the stage of microbial reproduction by inhibiting the biosynthesis of cell wall mucopolypeptides. It has antibacterial effects on Gram-positive aerobic microorganisms (such as Staphylococcus aureus) and Gram-negative aerobic microorganisms (such as Escherichia coli, Haemophilus influenzae, etc.).

It has a β-lactam structure similar to that of penicillin, which can block the active site of β-lactamase, inactivate these enzymes produced by bacteria, and enhance the effect of amoxicillin against enzyme-producing strains.

It can dilate peripheral blood vessels, especially increase venous blood volume, reduce the amount of blood returning to the heart, reduce the preload and postload of the heart, and thus reduce myocardial oxygen consumption; at the same time, it can also improve the blood supply to the ischemic area by promoting the redistribution of myocardial blood flow, and may exert its anti-myocardial ischemia effect through these two aspects.

Isosorbide mononitrate is the main active metabolite of isosorbide nitrate in the body. It can dilate peripheral blood vessels, especially increase venous blood volume, reduce venous return, reduce cardiac preload and afterload, thereby reducing myocardial oxygen consumption; at the same time, it can also improve blood supply to the ischemic area by promoting myocardial blood flow redistribution, and may play an anti-myocardial ischemic role through these two aspects. The LD50 (mg/kg) of intravenous injection of isosorbide mononitrate is: 2250 (female) and 1820 (male) in mice; 1750 (female) and 1880 (male) in rats.