What is the role of virus preservation solution in nucleic acid detection?

The epidemic situation in Shijiazhuang, Hebei Province, let us see the attack of the epidemic again. For everyone's safety, nucleic acid detection is on again. When it comes to nucleic acid testing, we have to mention our virus preservation solution. What is the virus preservation solution? What does it do?

Virus preservation solution is a kind of protective liquid medium added in the virus sampling tube to protect the samples after nasopharyngeal swab sampling, which is usually called virus preservation solution in China. Usually, nucleic acid PCR can not be directly carried out at the sample collection place during nucleic acid detection, so it is necessary to add virus preservation solution when the swab samples are transported for inspection.

However, according to different detection conditions and requirements, virus preservation solution can be divided into inactivated and non inactivated.

Inactivated virus preservation solution:

1. It is mainly the improved virus cleavage preservation solution with high concentration of cleavage salt, which can quickly and efficiently inactivate the virus protein of the sample to be tested;

2. It can effectively prevent the operator from secondary infection;

3. At the same time, it also contains RNase inhibitor, which can protect the viral nucleic acid from degradation;

4. Moreover, it can be stored at room temperature for a relatively long time, saving the cost of virus sample storage and transportation.

Non inactivated preservation solution:

1. It is mainly a modified virus maintenance solution based on transport medium;

2. In this case, the protein coat of the virus and the nucleic acid DNA or RNA of the virus are preserved at the same time;

3. In this way, the virus has the integrity of protein epitope and nucleic acid in vitro;

4. Of course, there is a certain risk of infection when the operation is wrong. However, it keeps the original virus samples to the greatest extent, and it needs to be kept at a strict low temperature for a long time after sampling.

Two types of preservation solution are necessary, which is determined by different experimental purposes and different detection conditions. No matter which kind of virus preservation solution is, it has its advantages. When we choose, we should choose according to the actual situation. You may think that there is a certain risk in non inactivated preservation solution, but it is also very safe as long as protective measures are taken.