Guanidine Hydrochloride High-Purity Reagent for Protein Denaturation, Molecular Biology, and Industrial Applications

Guanidine Hydrochloride High-Purity Reagent for Protein Denaturation, Molecular Biology, and Industrial Applications

Chemical Name: Guanidine Hydrochloride
Synonyms: Guanidinium chloride, Guanidinium hydrochloride
CAS Number: 50-01-1
Molecular Formula: CH₆ClN₃
Molecular Weight: 95.53 g/mol
Appearance: White crystalline powder or granules
Purity: 99% min (ACS/Reagent grade available)

🔍 Product Overview:

Guanidine Hydrochloride is a strong protein denaturant and chaotropic agent widely used in biochemistry, molecular biology, and industrial processing. It is primarily used to disrupt hydrogen bonding in proteins and nucleic acids, facilitating the unfolding of secondary and tertiary structures. Due to its ability to solubilize cellular components, it is a key ingredient in RNA/DNA extraction kits, protein refolding protocols, and enzyme inactivation procedures.

In addition to laboratory and pharmaceutical uses, guanidine hydrochloride also finds roles in organic synthesis, textile processing, and electroplating solutions.

✅ Key Features and Benefits:

• ✔️ Powerful denaturant of proteins and nucleic acids

• ✔️ Ideal for RNA and DNA purification workflows

• ✔️ Used in protein refolding and enzyme inactivation procedures

• ✔️ Compatible with chaotropic buffer systems

• ✔️ High solubility in water

• ✔️ Available in laboratory and industrial grades

• ✔️ Stable, long shelf life, and easy to handle

🧪 Applications:

🔬 Biochemical and Molecular Biology:

• Protein denaturation and unfolding studies

• RNA/DNA extraction (especially viral RNA)

• Protein renaturation/refolding

• Enzyme inhibition or inactivation

• Western blotting and protein electrophoresis buffers

• Protein crystallography preparation

🏭 Industrial and Manufacturing:

• Intermediate in organic synthesis

• Additive in electroplating solutions

• Flame retardant formulations

• Textile dyeing and processing agent

• Raw material in the production of pharmaceuticals and polymers

⚙️ Chemical and Physical Properties:

📋 Typical Specification (Reagent Grade):

📦 Packaging & Storage:

• Available in 500 g, 1 kg, 5 kg, and 25 kg quantities

• Packaging: HDPE containers or double-lined fiber drums

• Shelf Life: 2 years from manufacture date when stored properly

• Storage Conditions: Keep tightly closed in a cool, dry place. Avoid moisture and direct sunlight.

• Labeling: Custom labels with batch number, expiry date, and purity data

⚠️ Safety and Handling:

• Hazards: May cause irritation to skin, eyes, and respiratory tract if inhaled or ingested

• Precautions: Use gloves, safety goggles, and lab coat during handling

• First Aid: Rinse immediately if in contact with skin or eyes. Seek medical attention if ingested or inhaled in large amounts

• Disposal: Dispose in accordance with local environmental regulations

• GHS Classification:

  • Signal word: Warning

  • Hazard statements: H315, H319, H335

🔁 Related Products:

• Guanidine Thiocyanate (for nucleic acid extraction)

• Urea (protein denaturant)

• Tris and EDTA buffers (molecular biology grade)

🏁 Conclusion:

Guanidine Hydrochloride is an essential tool in modern biochemical research and industrial processes, offering high efficacy as a chaotropic agent and a reliable reagent in molecular biology protocols. Its versatility, purity, and effectiveness make it a staple in laboratories and manufacturing settings worldwide.

🧬 Guanidine Hydrochloride – Denature, Disrupt, and Discover.

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