High Purity GHK-CU Skin Cell Research Freeze-Dried Lab Research Solid Reagent

  Product Detail  

Brief Overview

Core Research Application & Functional Features

1. Permitted Research Scope Only

• In-vitro fibroblast cell culture tests on extracellular matrix synthesis regulation
• Tissue repair and wound recovery related cell activity comparative trials
• Antioxidant balance and free radical clearance mechanism observation experiments
• Collagen, elastin and glycosaminoglycan secretion quantitative detection research
• Metalloproteinase activity control and skin tissue remodeling pathway study
• Anti-aging signal transduction and cell survival cycle academic biochemical projects
• Concentration gradient contrast experiments of copper ion transport inside cell models

2. Unique Research Advantages

1. Stable Copper Ion Binding Structure
   
   
   The molecular structure forms stable coordination binding with copper ions, avoiding free metal ion oxidation interference in cell culture environments. It can steadily deliver trace copper elements to intracellular reaction sites, providing accurate single-variable data for metal transport related lab tests.
2. Freeze-Dried Solid Form Long-Term Stability
   
   
   Low-temperature vacuum dehydration locks complete molecular binding structure without structural dissociation during storage and transportation. It effectively prevents activity attenuation issues existing in liquid medium samples, greatly reducing frequent re-preparation work for long-period lab stock management.
3. Wide Compatibility With Cell Culture Systems
   
   
   This solid can fully blend with standard lab bacteriostatic purified water and PBS buffer liquid, compatible with most conventional mammalian fibroblast, epithelial cell and tissue slice test systems, without introducing extra interfering impurities to cell culture environment.
4. Obvious Concentration Gradient Experimental Response
   
   
   Adjustable working concentration range fits diversified lab design; cell matrix secretion and antioxidant indicators show distinct gradient changes under different addition dosages, convenient for quantitative data statistics and academic paper result analysis.
5. Low Impurity Interference Profile
   
   
   Multi-stage precise purification workflow minimizes irrelevant residual substances inside finished solid. It will not generate extra noise signals in spectrophotometry and fluorescence detection equipment, ensuring high definition test data output for biochemical quantitative analysis.
6. Uniform Batch-to-Batch Consistency
   
   
   Standardized freeze-drying and purification production workflow ensures identical purity, dissolving performance and molecular binding activity across all production lots. No test result deviation caused by inconsistent raw material quality, fully fit for parallel comparison experiments and repeated result verification.

Complete Standard Specification Parameters

Basic Product Identification Info

• Full Product Name: GHK-CU Copper Complex (Copper Tripeptide-1)
• CAS Reference Number: 49557-75-7
• Molecular Formula: C₁₄H₂₄CuN₆O₄
• Theoretical Molecular Weight: 403.93 Da
• Visual Form: Uniform blue to deep blue freeze-dried solid, free of clumps, discoloration, tiny foreign particles or visible impurities
• Odor Characteristic: Completely odorless
• Dissolving Performance: Fully blendable with lab bacteriostatic purified water and PBS buffer liquid; cannot mix with alcohol-based lab liquid medium
• Post-dissolving Liquid State: Clear light blue transparent solution without suspended particles, turbidity or precipitate after full dissolution
• pH Value of 1mg/mL Mixed Liquid: 5.2 ~ 7.2
• Residual Moisture Content Test Index: ≤ 2.0% (tested via Karl Fischer titration method per batch)
• Endotoxin Level: < 0.1 EU/mg, fully comply with global universal safety standards for cell and animal lab research
• Heavy Element Residue Control Limit: Lead ≤10ppm, Arsenic ≤2ppm, Cadmium ≤1ppm, Mercury ≤1ppm
• Microbe Control Standard: Total aerobic microbes <10 CFU/mg, yeast and mold <5 CFU/mg; zero harmful pathogenic bacteria detected in all batches
• Molecular Structure Integrity: Spectrum matching test carried out for every batch to confirm complete intact copper complex molecular frame without breakage or dissociation

Available Sealed Vial Sizes (No bulk kilogram supply)

• GHK-50: 50mg freeze-dried copper complex solid per vacuum-sealed neutral glass vial
• GHK-100: 100mg freeze-dried copper complex solid per vacuum-sealed neutral glass vial

Strict Production & Inspection Quality Control Standards

1. Core High Purity Benchmark
   
   
   Guaranteed HPLC chromatographic purity of finished copper complex solid ≥99.0%. Complete spectrum chromatogram records are attached to each batch test report. Single unknown individual impurity ≤0.3%, total combined unknown impurities ≤1.0%. Testing adopts C18 reverse-phase chromatographic column with 220nm UV wavelength detection for precise purity numerical measurement.
2. Mandatory Full Batch Testing Items

• Spectrum molecular weight matching test, ensuring measured molecular weight error within ±5 Da compared with theoretical standard value
• Full molecular frame verification to confirm complete matching with standard GHK-CU copper complex structure
• Trace residual substance testing following ICH Q3C standardized limits
• Constant temperature & humidity accelerated stability test for shelf life effectiveness validation
• Copper complex binding activity detection to lock stable and consistent lab research performance
• 100% manual visual full inspection of all finished sealed vials before shipment to screen out defective goods
• Complete batch full traceability management system covering raw material incoming records, production operation logs, full-item inspection data and outgoing delivery archive files

Standard Step-by-Step Dissolving Operation Guidance

1. Slowly pour prepared liquid medium along the inner wall of the glass vial to avoid massive foaming generation
2. Fully tighten the vial cap, rotate and swirl gently in circular motion until all freeze-dried solid material dissolves completely
3. Do not shake the vial violently; fierce agitation will break complete molecular frame structure and weaken stable copper binding activity
   
   
   Suggested Working Concentration Range For Fibroblast Cell Model Research: 1nM – 1000nM, adjustable flexibly based on customized experiment design and cell strain species

Storage Environment & Valid Usage Period Rules

Unopened Original Vacuum-Sealed Vials

• Long-term storage environmental condition: Keep fully sealed vials stored at -20°C ~ -80°C, completely shielded from direct light and high moisture
• Valid storage duration under constant -20°C freezing environment: 12 months
• Valid storage duration under constant -80°C ultra-low temperature environment: 36 months
• Short transit stability tolerance: Maintain stable molecular binding activity for up to 7 days under ambient temperature below 25°C
• Forbidden storage behavior: Frequent repeated temperature fluctuation, long-time direct sunlight exposure

Liquid Mixed With Matching Purified Water

• Storage condition after dissolution: Place inside refrigerator under 2°C ~ 8°C refrigeration environment, fully shaded
• Suggested full usage time limit: Consume all mixed liquid within 14 days after preparation completion
• Strict prohibition: Do not freeze diluted mixed liquid; repeated freezing and thawing cycles will permanently damage copper complex molecular stability and lose biological activity

Shipping & Receiving Handling Reminders

1. Small trial vial orders can be delivered via standard international express for short-distance cross-border transit
2. Large quantity multi-vial orders are strongly suggested to choose temperature-insulated protective packaging to maintain stable molecular state during long-distance overseas transportation
3. Complete supporting shipping documents will be delivered together with all parcels, including unified technical data sheets and batch exclusive test records for customs inspection and internal lab filing use

Lab Operation Safety Reminders

1. Wear disposable nitrile protective gloves, transparent safety goggles and dust-proof lab masks during powder weighing and dissolving operating procedures
2. Avoid direct skin contact, eye contact and inhalation of dry freeze-dried copper complex solid powder
3. In case of accidental contact with skin or eye tissue, flush the affected area thoroughly with plenty of clean running water immediately
4. Dispose of residual solid powder and waste mixed liquid strictly following internal waste management rules of your laboratory; do not pour residual liquid directly into domestic drainage pipelines or general household waste bins

Important Usage Restriction Statement

Free Supporting Technical Documents Provided For All Orders

1. Batch exclusive test report with full HPLC spectrum purity test data records
2. Full-version unified technical data sheet covering all specifications, dissolving operation steps and standard storage rules
3. Complete molecular structure schematic diagram and structure verification file
4. Full inspection records of heavy element, endotoxin and microbe testing indexes
5. Original raw data of long-term molecular stability accelerated testing
   
   
   Customized lab dedicated experiment operation guidance documents can be provided upon customer request for institutional internal research archive sorting and lab safety training use
6.