 Home > Encyclopedia > Pectinase

Pectinase

Pectinase structure

CAS No:
9032-75-1
Formula:
C18H37N(CH3)2
Chemical Name:
Pectinase
Synonyms:
POLYGALACTURONASE PECTINASE;PECTINASE FROM RHIZOPUS SP;PECTINASE FROM RHIZOPUS SP. PLANT CELL*C ULTURE TEST;PECTINASE FROM ASPERGILLUS NIGER -*PLANT CELL CULT;PECTINASE FROM ASPERGILLUS NIGER;Pectinase from Aspergillus niger, ~1 U/m g;PECTINASE FROM RHIZOPUS SP. ~20 U/MG;POLYGALACTURONASE, STERILE, FOR PLANT*TI SSUE CULTUR
Categories:
Food Additives > Enzyme

Description

Pectinase is a complex enzyme consisting of pectolyase, pectozyme, and polygalacturonase. It is able to break down pectin, a kind of polysaccharide existing in plant cell wall. Pectin is It functions optimally at temperatures between 45 and 55 °C and pH between 3 and 6.5. Owing to this property, Pectinase is often used in processes demanding the degradation of plant materials such as speeding up the extraction process of fruit juice. More importantly, through breaking down pectin, which is an important diet component of human being, pectinase can positively affect the digestive system, maintain intestinal bacteria balance and promote colon health. Pectinase is also useful during the production of wine for aiding the extraction of flavours from the mash. Pectinase is also used for retting. It is usually obtained from the fermentation of fungi such as Aspergillus Niger.

It is usually an off-white powder, or a brownish yellow liquid. There are three main enzymes that are active ingredients in commercial pectinases. One is pectin pectylhydrolase (EC 3.1.1.11), whose main function is to catalyze methyl ester pectin in order to remove the methyl ester group to produce polygalacturonide bonds and methanol. Another is polygalacturonase (Polygalacturonase; EC 3.2.1.15) whose role is to hydrolyze the α-1,4-bonded galacturonide groups in pectin to reducing sugars. The third is pectin lyase (EC 4.2.2.10), which breaks pectin to yield oligosaccharides. In addition, β-Glucanase (β-Glucanase; EC 3.2.1.6), β-Glucosidase (β-Glucosidase; EC 3.2.1.21) and Xylanase (Xylanase; EC 3.2.1.32) play a secondary role. Acting temperature 40～50°C, optimum pH 3.5～4.0. Iron, copper and zinc ions have obvious inhibitory effects. Soluble in water. Natural products are widely found in higher plants (e.g. citrus, apple, tomato, etc.) and microorganisms.

This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research.

Pectinase Basic Attributes

Molecular Weight：

Exact Mass：

150.052826

EC Number：

232-885-6

TSCA：

TSCA listed

Color/Form：

slightly beige

HScode：

35079090

Characteristics

Physical Properties：

The molecular weight of pectinase is generally between 20kD and 60kD, and most of them exist as monomeric enzymes. Pectinase activity is usually in the range of pH 3.0 to 9.0, with an isoelectric point of 4.0 to 9.0. Among them, an optimum pH of a hydrolase is 4.0 to 6.5, and its action does not require the participation of Ca2+. The optimum pH of the penctin lyase is 8.0 to 10.0, and its action requires Ca2+ involvement. The pectinase produced by different strains has different properties. Common purification methods of pectinase include ammonium sulfate precipitation, acetone precipitation, ion exchange chromatography and gel filtration chromatography.

XLogP3：

-2.39

Density：

1.5±0.1 g/cm3

Boiling Point：

415.5±38.0 °C at 760 mmHg

Flash Point：

219.2±23.3 °C

Refractive Index：

1.544

Water Solubility：

Soluble in water and practically insoluble in ethanol and ether.

Critical Temperature & Pressure：

2-8°C

Safety Information

Transport codes：

NONH for all modes of transport

wgk germany：

safety instructions：

24/25

Pectinase Use and Manufacturing

Pectinase, Aspergillus niger is used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase helps facilitate the maceration of plant materials and extraction processes including organelle preparation. It is used in the enzyme preparation. Used mostly to reduce viscosity, to improve filtration to clarify products, to avoid particle sedimentation and to prevent pectin gel formation in the manufacture of fruit juice and wine.

Pectinase fromRhizopus sphas been used for enzymatic digestion of leaves.

Material

Glucose

Downstream Products

Pectin

Recommended Suppliers of Pectinase

SHANDONG LOOK CHEMICAL CO.,LTD Contact Supplier

CN
5 YRS

Business licensed Certified factory

Manufactory Supplier of Agrochemicals,Daily Chemicals,Catalysts & Chemical Auxiliary Agents,Extract,Inorganic Chemicals,Organic Intermediate,Pigment & Dyestuff,Polymer,Flavour & Fragrance,Basic Organic Chemicals,Pharmaceutical

Pectinase liquid 30000 u/g QSJ-9032-75-1

CAS No.: 9032-75-1

Grade: Food Grade

Inquiry
HANGZHOU LEAP CHEM CO., LTD. Contact Supplier

CN
5 YRS

Business licensed

Trader Supplier of Intermediates,Building blocks,API,Silicones,Peptides,Lab chemicals,Biochemicals,Pharmaceuticals,Screening Compounds,Food Additives

Pectinase

CAS No.: 9032-75-1

Content: 99.00%

Inquiry
M A M M TRANDING ENTERPRISE Contact Supplier

ZA
6 YRS

Business licensed

Trader Supplier of chemical

Pectinase Enzyme Pectinase 99% Powder SAA09989DS6172 SAA

Unit Price: $9800-11000 /MT FOB

CAS No.: 9032-75-1

Grade: Food Grade

Content: 99%

Inquiry
Creative Enzymes Contact Supplier

US
6 YRS

Business licensed Certified factory

Manufactory Supplier of Coenzymes,Inhibito,Enzymes,Zymogens,Substrates

Alkaline pectinase for Bio-Scouring

CAS No.: 9032-75-1

Inquiry
Henan Lihao Chem Plant Limited Contact Supplier

CN
5 YRS

Business licensed

Trader Supplier of PVC resin,pvc paste resin,melamine

Macerozyme R-10 99% white powder kanbei

CAS No.: 9032-75-1

Grade: Industrial Grade

Content: 99%

Inquiry