5′-CTP

Solid

Solid

CTP is a pyrimidine ribonucleoside 5'-triphosphate and a cytidine 5'-phosphate. It has a role as an Escherichia coli metabolite and a mouse metabolite. It is a conjugate acid of a CTP(4-) and a CTP(3-).|Cytidine Triphosphate is a cytosine derived nucleotide comprised of three phosphate groups esterified to the deoxyribose sugar moiety. Nucleotide metabolism is upregulated in cancer cells; therefore, this nucleotide could be used as a biomarker to detect the presence of cancer cells.|Cytidine 5'-(tetrahydrogen triphosphate). A cytosine nucleotide containing three phosphate groups esterified to the sugar moiety.

Fermentation with CTP as raw material, remove protein, take 6.5kg of potassium dihydrogen phosphate, add appropriate amount of water to dissolve, add 0.6kg of magnesium chloride, 17.5kg of glucose, stir to dissolve, take 2.5kg of CMP and dissolve in water, adjust pH with sodium hydroxide =7, add water to 70L, heat to 50-60℃ with stirring, add 180L of yeast liquid, keep it at 30-32℃ for 3h, add hydrochloric acid to the end of the reaction to adjust pH=5, stop the reaction. Add trichloroacetic acid, adjust pH=2.5, centrifuge to obtain clear liquid. CMP[Yeast]→[30-32℃, 3h] fermentation broth [trichloroacetic acid]→[pH2.5] supernatant desalting, precipitation, purification, adding the supernatant to strong acid styrene cation exchange resin (001× 7) Stir and absorb for 15min, make the pH 1.5-1.7, let stand for 30min, filter, add ethanol to the filtrate until the alcohol concentration is 75%, let stand overnight, filter, add water to dissolve the filter cake to make the concentration 25%, then add five One part of the filter cake volume of diatomaceous earth, stir and filter, and collect the filtrate. Supernatant [001×7 resin]→[pH1.5-1.7, 15min] filtrate [ethanol]→[8h] precipitate [water, diatomaceous earth]→[stirring, filtration] filtrate adsorption, elution, decolorization The filtrate is diluted with water into a 0.5%-0.6% solution, adjusted to Ph=2-2.5, after adsorbing with a strong alkaline quaternary ammonium type I anion exchange resin (717), first washing with water for 8h, and then using 0.03mol/L of pH 2 Sodium chloride solution eluted ADP. When the absorbance of the solution at the wavelength of 280nm was greater than 0.4, wash with water at pH 2 for 4 hours, and finally eluted ATP with 1mol/L sodium chloride solution at pH 2, and the eluent was added to 1/1000 The amount of activated carbon is stirred, decolorized, filtered, and the filtrate is collected. Filtrate [717 resin]→[pH2-2.5] adsorbent [sodium chloride]→[pH2] elution [activated carbon]→[40min] filtrate precipitation, dry the filtrate to adjust Ph=3-5, add 3 times the amount of ethanol, static Set, filter, wash the filter cake with absolute ethanol and anhydrous ether, and dry under vacuum to obtain disodium cytidine triphosphate. The filtrate [ethanol]→[pH3.5] precipitate [anhydrous ethanol, anhydrous ether]→disodium cytidine triphosphate.

It is suitable for the adjuvant treatment of fatty liver, cerebral nerve diseases and atherosclerosis.

Molecular Weight:483.16
XLogP3:-5.6
Hydrogen Bond Donor Count:7
Hydrogen Bond Acceptor Count:14
Rotatable Bond Count:8
Exact Mass:482.98451319
Monoisotopic Mass:482.98451319
Topological Polar Surface Area:268
Heavy Atom Count:29
Complexity:855
Defined Atom Stereocenter Count:4
Covalently-Bonded Unit Count:1
Compound Is Canonicalized:Yes