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Nitro Blue Tetrazolium

Nitro Blue Tetrazolium structure

CAS No:
298-83-9
Formula:
C40H30N10O6.2Cl
Chemical Name:
Nitro Blue Tetrazolium
Synonyms:
2H-Tetrazolium,2,2′-(3,3′-dimethoxy[1,1′-biphenyl]-4,4′-diyl)bis[3-(4-nitrophenyl)-5-phenyl-,chloride (1:2);2H-Tetrazolium,3,3′-(3,3′-dimethoxy-4,4′-biphenylylene)bis[2-(p-nitrophenyl)-5-phenyl-,dichloride;2H-Tetrazolium,2,2′-(3,3′-dimethoxy[1,1′-biphenyl]-4,4′-diyl)bis[3-(4-nitrophenyl)-5-phenyl-,dichloride;Tetrazolium,3,3′-(3,3′-dimethoxy-4,4′-biphenylylene)bis[2-(p-nitrophenyl)-5-phenyl-2H-chloride];2,2′-Bis(p-nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-biphenylylene)ditetrazolium chloride;3,3′-(3,3′-Dimethoxy-4,4′-biphenylylene)bis[2-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride];3,3′-(3,3′-Dimethoxy-4,4′-diphenylene)bis[2-(p-nitrophenyl)-5-phenyltetrazolium chloride];2,2′-Di-p-nitrophenyl-5,5′-diphenyl-3,3′-bis(3,3′-dimethoxy-4,4′-biphenylene)ditetrazolium chloride;2,2′-Dinitrophenyl-5,5′-diphenyl-3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride;Nitro BT;Tetrazolium Nitro BT;Tetrazolium nitro blue;Nitro Blue Tetrazolium;Nitro Tetrazolium BT;p-Nitro blue tetrazolium;2,2′-Bis(p-nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride;Nitrotetrazolium Chloride Blue;Nitrotetrazolium blue;p-Nitrotetrazolium blue;Nitro Blue Tetrazolium chloride;NBT;p-Nitro blue tetrazolium chloride;NBT (dye);p-NBT;NTB;NSC 27622;Nitro TB;67740-22-1;83800-46-8;87714-63-4;121287-37-4;141102-30-9;562102-25-4;679787-05-4;867000-48-4;1401448-88-1;1422392-93-5;2158197-17-0
Categories:
Chemical Reagents > Organic Reagents

Description

NBT(Nitro BT;p-Nitrotetrazolium blue) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.

Nitro blue tetrazolium dichloride is an organic chloride salt having nitro blue tetrazolium(2+) as the counterion. It contains a nitro blue tetrazolium(2+).|Colorless to yellow dye that is reducible to blue or black formazan crystals by certain cells; formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.

Nitro Blue Tetrazolium Basic Attributes

Molecular Weight：

817.64

Exact Mass：

816.172668

BRN：

4115923

EC Number：

206-067-4

UNII：

X44P41F7ZK

DSSTox ID：

DTXSID6046719

HScode：

32041900

Characteristics

PSA：

179

XLogP3：

1.29040

Appearance：

Yellow Powder/Solid

Density：

1.5521 (rough estimate)

Melting Point：

200°C

Refractive Index：

1.7350 (estimate)

Water Solubility：

soluble in water, ethanol and methanol.methanol: 50 mg/mL, clear, very strongly yellow

Storage Conditions：

2-8°C

Toxicity：

LD50 orl-mus: 2 g/kg YAKUD5 32,614,1990

Safety Information

Transport codes：

NONH for all modes of transport

wgk germany：

hazard class code：

22-68/20/21/22-20/21/22-36-36/37/38-41

safety instructions：

36-45-36/37-26-39

RTECS Number：

XF8045000

Marks：

Xn,Xi

Stability/Shelf Life：

Stable, but store cold.

Precautionary statement(s)：

P261-P280-P304 + P340 + P312-P337 + P313-P501

Hazard Statements：

H302

Drug Information

MeSH：

Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant and Hackh's Chemical Dictionary, 5th ed, p301, p499) (See all compounds classified as Indicators and Reagents.)

MeSH Entry Terms：

Blue, Nitrotetrazolium

Nitro Blue Tetrazolium Use and Manufacturing

Uses

Substrates for dehydrogenases and other peroxidases. It is used to determine glucose-6-phosphate dehydrogenase; it is also a precipitation reagent for clotting factor I (fibrinogen). It is also one of the substrates of alkaline phosphatase, which produces an insoluble blue product under the catalysis of alkaline phosphatase. Commonly used in immunohistochemistry (IHC), immunocytochemistry (ICC) and Western blot (WB) experiments.

Manufacturing Info：

2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, chloride (1:2): ACTIVE

Computed Properties

Molecular Weight:817.6
Hydrogen Bond Acceptor Count:12
Rotatable Bond Count:9
Exact Mass:816.1726841
Monoisotopic Mass:816.1726841
Topological Polar Surface Area:179
Heavy Atom Count:58
Complexity:1180
Covalently-Bonded Unit Count:3
Compound Is Canonicalized:Yes

Material

Benzaldehyde

Chlorine dioxide

Tetrahydrofuran

Ethanol

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