What is the relationship between nucleic acid detection and Tris and EDTA?

At present, the new crown virus has swept the world, which has a serious impact on human daily life. Nucleic acid detection is a new detection method of crown virus. In the specific process of nucleic acid detection, nucleic acid extraction is a very important link. According to the public reports, Tris, EDTA and other reagents play an important role in the extraction of nucleic acids.

Nucleic acid is a kind of biological macromolecule compound which is polymerized by many nucleotides, including DNA and RNA, which is one of the most basic substances in life. Nucleic acid extraction is a process of separating nucleic acids from samples by physical and chemical methods. It is a prerequisite technology for a series of molecular biological analysis such as nucleic acid amplification, DNA fragment connection, carrier construction, high-throughput sequencing, and is a very important technology in life science research, biological timely application and gene diagnosis. Therefore, the extraction of nucleic acid has very important meaning Righteousness.

Generally speaking, nucleic acid extraction is a multi-step work. Firstly, it is necessary to break the biological sample materials such as cells and tissue materials, inactivate nuclease and release nucleic acid. The role of Tris, EDTA and other reagents is mainly reflected in the release of nucleic acid.

Nucleic acids are easy to hydrolyze in acid solution and stable in neutral or weak alkali solutions. Tris, namely trimethylaminomethane, has a pH buffer range of 7.0-9.0, which can maintain the stability of the nucleic acid released after the sample is cracked, so as to avoid the degradation of nucleic acid and improve the concentration and purity of nucleic acid.

EDTA, which can be combined with mg2+, ca2+, mn2+, fe2+, can prevent metal ions from activating protease, thus reducing the influence of metal ions on nucleic acid quality.

In conclusion, Tris, EDTA and other reagents can fully and effectively crack cells in the process of nucleic acid extraction, so that the nucleic acids in the cells can be released fully and get the nucleic acids with higher concentration, which is conducive to improving the quality of nucleic acids and ensuring the accuracy of subsequent operations.

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