With the development of science and technology, the emergence of various advanced instruments, clinical biochemical projects for blood collection and separation put forward higher requirements, it not only requires the rapid results, but also requires us to ensure the accuracy of the results of each project. In the past, hospitals used to use ordinary drying tubes to separate blood samples. Recently, due to the development of related technology, many hospitals began to use heparin lithium anticoagulant tubes and separation gel coagulation promoting tubes to separate blood samples, and then centrifuged to determine and analyze the plasma (serum). What are the advantages of these two kinds of vacuum blood collection vessels?
Blood collection from the morning to the laboratory computer determination, generally placed at room temperature for 2-3 hours, during which the metabolic activity of cells did not stop, with the extension of storage time, intracellular and extracellular substances transfer. Serum separation gel is a kind of viscous fluid with specific gravity of 1.05, which is between serum (1.02) and blood clot (1.08). Its structure contains a lot of hydrogen bonds. Due to the association of hydrogen bonds, it forms a network structure. This kind of viscous liquid with network structure has thixotropy. When the separation gel and coagulated blood are centrifuged in the same test tube, the network structure will change under the action of centrifugal force The blood clot heavier than the separation glue is transferred to the bottom of the test tube, and the separation glue forms a colloidal isolation layer between the serum and the blood clot, blocking and reducing the channels of blood cells affecting the serum components, so as to play a role in stabilizing the substance in the serum.
However, heparin lithium tube anticoagulation can maintain the integrity of cells, delay the release of myocardial enzymes from red blood cells, and the separation of blood cells after centrifugation has a certain relationship with serum.
Therefore, the determination of myocardial zymogram is greatly affected by the release of substances in red blood cells. The separation rubber tube and heparin lithium tube have the advantages of rapid release of serum or plasma, less error caused by sample placement, less hemolysis, less impact on the results, and more real results. Relatively speaking, the separation hose can separate blood cells from serum, which makes the result closer to the condition of blood collection. Therefore, if conditions permit, we should try our best to use separation rubber tube or heparin lithium tube to separate myocardial enzyme samples. If we use ordinary drying tube to separate myocardial enzyme samples, we should separate serum as soon as possible after blood collection.
