Rhamnocitrin CAS 90-19-7 Research Grade Flavonoid Standard for Natural Product Analysis and Biological Activity Studies

• • • • Product Overview

        Rhamnocitrin (CAS 90-19-7) is a naturally occurring flavonoid compound belonging to the flavone subclass. It is a high‑purity research-grade reference standard isolated from various plant sources, particularly species of the genus Rhamnus and Citrus. The chemical structure features a 3,5,4′‑trihydroxy‑7‑methoxy flavone skeleton, which is characteristic of O‑methylated flavonoids. The compound exhibits a bright yellow crystalline appearance and demonstrates significant biological activities including antioxidant, anti‑inflammatory, and anticancer properties. As a reference standard, it is essential for the identification and quantification of rhamnocitrin in plant extracts and finished products.

        Synthetic Process / Isolation Method

        Rhamnocitrin is typically isolated from natural plant sources through a series of extraction and purification steps:

        • Extraction: Dried plant material (such as leaves or fruits) is extracted with organic solvents like ethanol or methanol.

        • Partitioning: The crude extract undergoes liquid‑liquid partitioning to concentrate the flavonoid fraction.

        • Chromatographic Purification: The concentrated extract is subjected to column chromatography using silica gel or Sephadex LH‑20, eluting with solvent systems such as chloroform‑methanol or ethyl acetate‑methanol.

        • Recrystallization: The purified compound is recrystallized from appropriate solvent systems to achieve ≥98% purity.

        • Quality Confirmation: The final product is rigorously characterized by HPLC, NMR (¹H‑NMR, ¹³C‑NMR), and mass spectrometry to confirm structural identity and purity.

        Mechanism of Action

        As a flavonoid compound, rhamnocitrin exerts its biological activities through multiple mechanisms:

        • Antioxidant Activity: The phenolic hydroxyl groups effectively scavenge free radicals and chelate metal ions, protecting cells from oxidative stress.

        • Anti‑inflammatory Activity: Modulates the NF‑κB signaling pathway and reduces the production of pro‑inflammatory cytokines such as TNF‑α and IL‑6.

        • Anticancer Potential: Induces apoptosis in cancer cells through the activation of caspase cascades and modulation of the PI3K/Akt pathway.

        • Cardioprotective Effects: Improves endothelial function and reduces oxidative damage to vascular tissues.

        Quality Specifications

        Parameter	Specification	Test Method
        Appearance	Yellow crystalline powder	Visual inspection
        Assay (Purity)	≥98.0%	HPLC area normalization
        Melting Point	290‑292 °C (dec.)	Capillary method
        Identification	Conforms to structure	¹H‑NMR, ¹³C‑NMR, MS
        Water Content	≤1.0%	Karl Fischer titration
        Residue on Ignition	≤0.1%	Gravimetric analysis
        Heavy Metals	≤20 ppm	ICP‑MS / AAS
        Shelf Life	24 months from date of manufacture	When stored under recommended conditions

        Packaging & Shipping

        • Packaging: Available in 1mg amber glass vials with PTFE‑lined caps, purged with inert gas to ensure stability.

        • Shipping conditions: This product is not classified as dangerous goods under international transport regulations. Can be shipped at ambient temperature for routine orders.

        • Storage conditions: Store at 2‑8°C, protected from light and moisture for long‑term stability.

        • Documentation: Each shipment includes a comprehensive Certificate of Analysis (COA), MSDS, and commercial invoice.

          Parameter	Specification	Details
          Product Name	Rhamnocitrin	Flavonoid reference standard
          Synonyms	7‑Methylkaempferol	—
          CAS Number	90‑19‑7	90‑19‑7
          Molecular Formula	C₁₆H₁₂O₆	16 carbons, 12 hydrogens, 6 oxygens
          Molecular Weight	300.26 g/mol	Exact mass: 300.0634
          Appearance	Yellow crystalline powder	Visual inspection
          Assay (Purity)	≥98.0%	HPLC area normalization
          Melting Point	290‑292 °C (dec.)	Capillary method
          Chemical Class	Flavonoid	O‑Methylated flavone
          Source	Rhamnus spp., Citrus spp.	Natural product isolation
          Storage Condition	2‑8°C, protected from light, sealed, dry	Store in a cool, dry place
          Shelf Life	24 months	From date of manufacture
          Packaging	1mg/vial	Amber glass vial
          Grade	Research Grade / Reference Standard	For laboratory and analytical use
          Application	Natural product analysis, biological activity studies, content determination	Reference standard for HPLC

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