 Home > Encyclopedia > Basic Orange 14

Basic Orange 14

Basic Orange 14 structure

CAS No:
10127-02-3
Formula:
C17H20Cl3N3Zn
Chemical Name:
Basic Orange 14
Synonyms:
Acridine Orange,pure,ca. 55% dye content;Acridine Orange hemi;Acridine Orange hemi(zinc chloride) salt, 1mg/mL;Acridine Orange Euchrysin GNY;ACRIDINE ORANGE ZINC CHLORIDE DOUBLE SAL;N,N,N',N'-tetramethylacridine-3,6-diamine monohydrochloride, compound with zinc dichloride;Acridine orange zinc chloride double salt (C.I.46005) for microscopy Certistain;ACRIDINE ORANGE HEMI(ZINC CHLORIDE)
Categories:
Dyes and Pigments > Dye

Description

brown powder

Acridine orange is a metachromatic fluorescent cationic dye that permeates the cell membrane and intercalates DNA and RNA. It allows for visual detection of nucleic acids on agarose and polyacrylamide gels.

Basic Orange 14 Basic Attributes

Molecular Weight：

438.1

Exact Mass：

736.135986

BRN：

3734978

EC Number：

233-353-6

Color/Form：

Red brown

HScode：

32041300

Characteristics

λmax：

489 nm

PSA：

19.4 Å²

Appearance：

Red brown Solid

Melting Point：

165 °C

Boiling Point：

468.6°C

Flash Point：

237.2ºC

Water Solubility：

soluble

Storage Conditions：

Store at RT.

Toxicity：

A dye used to stain nucleic acids. It fluoresces at 530 nm when intercalated into double stranded DNA, or at 640 nm when ionically bound to single stranded DNA. It produces mutations, some involving reading frame shifts, other deletions or insertions and is carcinogenic. It is also known to be a dermal phototoxicant.

PH：

3.9 (10g/l, H2O, 20℃)

PKA：

pKa -3.3(H2O T=room temperature(RT) Iunspecied) (Uncertain);0.2(H2O T=room temperature(RT) Iunspecied) (Uncertain);10.1(H2O T=room temperature(RT) Iunspecied) (Uncertain)

Critical Temperature & Pressure：

room temp

Safety Information

Packing level：

Ⅲ

Hazard category：

6.1

Transport codes：

2811

wgk germany：

hazard class code：

Xn,Xi

safety instructions：

45-36/37-36-26

RTECS Number：

AR7600000

Marks：

Xn,Xi

Precautionary statement(s)：

P281

Hazard Statements：

68-20/21/22-36/37/38

GHS Classification：

|Warning|H341 (97.83%): Suspected of causing genetic defects [Warning Germ cell mutagenicity]|P201, P202, P281, P308+P313, P405, and P501|Aggregated GHS information provided by 46 companies from 4 notifications to the ECHA C&L Inventory.

Basic Orange 14 Use and Manufacturing

Used as a fluorescent indicator, mutagen for tumor cells and bacteria, nucleic acid stains and frameshift mutations. Acridine orange is a fluorescent pigment. The wavelength of the acridine orange excitation filter is 488 nm. The blocking filter wavelength is 515nm. Acridine orange has a difference in the amount of DNA and RNA binding in the cell, and can emit different colors of fluorescence (that is, coloring specificity). This is because DNA is a highly polymer, and the location of absorbing fluorescent substances is less, and it emits green fluorescence, while RNA The degree of polymerization is low, and there are many positions that can be combined with the fluorescent substance, so it emits red fluorescence.

Fluorescent stain for nucleic acids. An RNA polymerase inhibitorAcridine Orange acts as a lysosomal dye. It is used for cell-cycle studies. It plays an important role as a nucleic acid-selective fluorescent cationic dye, which is useful for cell cycle determination. Further, it serves as an inhibitor of RNA polymerase.

Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.

Computed Properties

Molecular Weight:438.1
Hydrogen Bond Donor Count:1
Hydrogen Bond Acceptor Count:5
Rotatable Bond Count:2
Exact Mass:435.001423
Monoisotopic Mass:435.001423
Topological Polar Surface Area:19.4
Heavy Atom Count:24
Complexity:298
Covalently-Bonded Unit Count:5
Compound Is Canonicalized:Yes

Recommended Suppliers of Basic Orange 14

HANGZHOU LEAP CHEM CO., LTD. Contact Supplier

CN
5 YRS

Business licensed

Trader Supplier of Intermediates,Building blocks,API,Silicones,Peptides,Lab chemicals,Biochemicals,Pharmaceuticals,Screening Compounds,Food Additives

Basic Orange 14

CAS No.: 10127-02-3

Content: 99.00%

Inquiry
Jinan topfull chemical co.,ltd Contact Supplier

CN
1 YR

Business licensed

Trader Supplier of 3-(1-Naphthoyl)indole

Basic Orange 14 Electrophoresis Grade Dye CAS 10127-02-3 Research Grade Biological Stain

Unit Price: $70-80 /G FOB

CAS No.: 10127-02-3

Grade: Reagent Grade

Content: 99%

Inquiry