5-Cyclopropyl-2-[1-[(2-fluorophenyl)methyl]-1H-pyrazolo[3,4-b]pyridin-3-yl]-4-pyrimidinamine

103.7 mg (0.38 mmol) of the amidine from Example VIII and 168.1 mg (1.54 mmol) of the crude 2-cyclopropyl-3-oxopropionitrile from Example I are added together in 0.5 ml of toluene and mixed in an ultrasonic bath. The toluene is evaporated under reduced pressure and the mixture is heated to 100-105° C. without solvent in an open vessel. After one hour, the mixture is cooled, the residue is dissolved in dichloromethane and admixed with 1 g of silica gel and the mixture is concentrated using a rotary evaporator. For purification, the substance is chromatographed over silica gel 60 (particle size 0.040-0.063 mm) using cyclohexane/ethyl acetate 1:1 as mobile phase. Yield: 50.0 mg (36.0percent of theory).

BAY 41-2272 is a soluble guanylate cyclase agonist that activates human mononuclear phagocytes. Inhibits vascular smooth muscle growth through the cAMP-dependent protein kinase and cGMP-dependent protein kinase pathways.