Triphenyltetrazolium chloride
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Triphenyltetrazolium chloride
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CAS No:
298-96-4
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Formula:
C19H15N4.Cl
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Chemical Name:
Triphenyltetrazolium chloride
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Synonyms:
2H-Tetrazolium,2,3,5-triphenyl-,chloride (1:1);2,3,5-Triphenyl-2H-tetrazolium chloride;2H-Tetrazolium,2,3,5-triphenyl-,chloride;PTB;Red tetrazolium;RT;TPTZ;2,3,5-Triphenyltetrazolium chloride;Triphenyltetrazolium chloride;TTC;Vitastain;Tetrazolium chloride;TT;Uroscreen;1,3,5-Triphenyl-2H-tetrazolium chloride;1,3,5-Triphenyltetrazolium chloride;Urocheck;Tetrazolium red;TTC (dye);52230-05-4;80949-00-4
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CAS No:
Description
Tetrazolium Red(2,3,5-Triphenyltetrazolium chloride; TPTZ) is used to visualize dehydrogenase enzyme activity; initially the tetrazolium solution is colorless but changes to red when it comes into contact with hydrogen. Tetrazolium red is used in a biochemical viability test for seeds. The test relies on dehydrogenase enzymes to release hydrogen ions which subsequently reduce the colorless tetrazolium salt solution to a red compound called formazan. Living cells turn red while dead cells
2,3,5-triphenyltetrazolium chloride is an organic chloride salt having 2,3,5-triphenyltetrazolium as the counterion. It has a role as an indicator and a dye. It contains a 2,3,5-triphenyltetrazolium.
Triphenyltetrazolium chloride Basic Attributes
334.80200
334.09900
206-071-6
D25727K0RB
5053
2933990090
Characteristics
34.59000
0.21500
White to yellow with a light tan cast powder
1.1704 (rough estimate)
243 °C (decomp)
>360
91.7ºC
H2O: may be clear to slightly hazy
2-8ºC
0.0615mmHg at 25°C
LD50 intravenous in mouse: 5600ug/kg
Safety Information
NONH for all modes of transport
3
R36/37/38
S26-S36
XF8100000
Xi
Stable. Light sensitive. Protect from moisture. Incompatible with strong oxidizing agents.
P261-P305 + P351 + P338
H315-H319-H335
|Warning|H315 (100%): Causes skin irritation [Warning Skin corrosion/irritation]|P261, P264, P271, P280, P302+P352, P304+P340, P305+P351+P338, P312, P321, P332+P313, P337+P313, P362, P403+P233, P405, and P501|Aggregated GHS information provided by 59 companies from 9 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies.
Drug Information
Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS. (See all compounds classified as Coloring Agents.)
2,3,5-triphenyltetrazolium chloride
Triphenyltetrazolium chloride Use and Manufacturing
E. Mixing the solid 2, 3, 5-triphenylformamidine IV-1 obtained in step D with 300 mL of dichloromethane and 300 mL of waterAfter the solvent was dissolved, N-chlorosuccinimide (0.11 mol, 14.7 g) was added, and the reaction was carried out at 0 ° C for 5 h;F. The mixed solution in step E is allowed to stand for stratification, the upper layer (aqueous solution) is taken out, and the solvent is distilled off to obtain 2, 3, 5-triphenyltetrazole compound V-1 was purified by recrystallization from chloroform to give 30.2 g (yield: 90percent).HPLC Method E Hibar RT 125-4, 5.0 mum, 4.0*125 mm; gradient elution, 5% to 80% solvent B (0.1% TFA in acetonitrile) in solvent A (0.1% TFA in water) within 10 min, 2.0 ml/min, detection at 214 nm, temperature 35 C.HPLC method E Hibar RT 125-4, 5.0 mum, 4.0*125 mm; gradient elution, 5% to 80% solvent B (0.1% TFA in acetonitrile) in solvent A (0.1% TFA in water) within 10 min, 2.0 ml/min, detection at 214 nm, temperature 35 C.E. Mixing the solid 2, 3, 5-triphenylformamidine IV-1 obtained in step D with 300 mL of dichloromethane and 300 mL of waterAfter the solvent was dissolved, N-chlorosuccinimide (0.11 mol, 14.7 g) was added, and the reaction was carried out at 0 C for 5 h;F. The mixed solution in step E is allowed to stand for stratification, the upper layer (aqueous solution) is taken out, and the solvent is distilled off to obtain 2, 3, 5-triphenyltetrazole compound V-1 was purified by recrystallization from chloroform to give 30.2 g (yield: 90%).
Substance is used in analytical chemistry as a sensitive reagent for reducing sugars, and to distinguish between ketols and simple aldehydes.
2H-Tetrazolium, 2,3,5-triphenyl-, chloride (1:1): ACTIVE
Computed Properties
Molecular Weight:334.8
Hydrogen Bond Acceptor Count:3
Rotatable Bond Count:3
Exact Mass:334.0985242
Monoisotopic Mass:334.0985242
Topological Polar Surface Area:34.6
Heavy Atom Count:24
Complexity:330
Covalently-Bonded Unit Count:2
Compound Is Canonicalized:Yes
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