LL-37, Human Cathelicidin Antimicrobial Peptide

• 1. Reconstitution & Dissolution

  
  
  1. Before use, centrifuge the vial briefly to collect all powder at the bottom.
  2. Add appropriate solvent: ultrapure water, dilute acetic acid or DMSO is recommended.
  3. Gently swirl or pipette up and down slowly until fully dissolved; do not shake vigorously to avoid peptide denaturation.
  4. Prepare working solution on demand. Avoid long-term storage of aqueous solution.
  
  

  2. Recommended Working Concentration

  
  
  • Antibacterial / Anti-biofilm test: 1 – 10 μM
  • Cell experiment (Immunomodulation / Wound healing): 0.5 – 5 μM
  • Skin care formulation: 0.1 – 2 μM (formulate per formula requirements)
  
  

  3. Dilution Steps

  
  
  Calculate the required solvent volume based on target concentration. Example for reference:

  
  

  Take 1 mg LL-37 (MW: 4493.33 Da)
  
  
  • Moles of 1 mg peptide: ≈ 2.2255×10⁻⁷ mol
  • To prepare 10 μM stock solution: add 0.022255 L (22.26 mL) solvent
  
  

  4. Storage After Dissolution

  
  
  • Stock solution: Aliquot and store at -80°C, valid for 3–6 months.
  • Avoid repeated freeze-thaw cycles.
  • Working solution: Use immediately after preparation.
  
  

  5. Operation Notes

  
  
  1. Wear gloves and masks during operation to prevent contamination.
  2. Do not use high-salt solution for initial dissolution, which may cause precipitation.
  3. Keep away from proteases to prevent peptide degradation.
  4. For in vitro research & formula use only. Not for direct human injection or oral administration.

1. Unopened Lyophilized Powder

1. Long-term storage: Seal tightly and keep away from light at -20°C. Shelf life: 24 months.
2. Temporary storage: Store at 15–25°C for no more than 7 days.
3. Environmental requirement: Keep dry, away from heat sources and strong light.

2. Reconstituted Stock Solution

1. Aliquot into single-use portions and freeze at -80°C. Valid for 3–6 months.
2. Avoid repeated freeze-thaw cycles to prevent activity loss and peptide degradation.
3. For short-term use (1–2 weeks): Keep sealed and shaded at 2–8°C.

3. Working Solution

4. Additional Notes

1. Centrifuge the vial briefly before opening to prevent powder scattering and moisture absorption.
2. Keep away from proteases and high-salt solutions to avoid inactivation.

Core Business & Product Portfolio

• Therapeutic peptides: SS-31 (Elamipretide), Tirzepatide, Semaglutide, Retatrutide (RT20), etc.
• Functional peptides: GHK-Cu, TB500, KPV, NAD, Glutathione, etc.
• Custom peptide services: Custom synthesis of complex sequences, long-chain peptides (up to 200 amino acids) and cyclic peptides, as well as various modification services (N-methylation, unusual amino acid incorporation, etc.).

R&D & Manufacturing Strength

• Technical expertise: Mastery of core technologies including solid-phase peptide synthesis (SPPS), liquid-phase synthesis, advanced purification (HPLC) and strict structural characterization.
• Quality assurance: All products are produced in compliance with cGMP standards, with complete CoA, HPLC and MS reports to ensure batch-to-batch consistency and high purity (≥98%).
• Production capacity: Equipped with professional production bases in Guangzhou, supporting small-batch R&D samples to large-scale commercial production, with stable supply capacity.

Global Market & Service Philosophy

Vision