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Home > Chemical Reagents > Organic Reagents > 3,3′,5,5′-Tetramethylbenzidine for Sale > 3,3',5,5'-Tetramethylbenzidine CAS 54827-17-7 TMB Reagent
3,3',5,5'-Tetramethylbenzidine CAS 54827-17-7 TMB Reagent buy - large image1
3,3',5,5'-Tetramethylbenzidine CAS 54827-17-7 TMB Reagent buy - image1

3,3',5,5'-Tetramethylbenzidine CAS 54827-17-7 TMB Reagent

Unit Price: Get Latest Price
CAS No.:

54827-17-7

Grade:

Industrial Grade

Content:

99%

Port:

shanghai

Brand:

Yacoo

Packaging:

1kg/bottle,25kg/drum

Price valid (until):

2025-04-27

Company Type:
Manufactory
Location:
China
Qualification:
Main Products:

tris,EDOT ,[2,2]Paracyclophane ,Guanidine hydrochloride ,Bovine albumin ,(3R,4R)-3,4-Dimethyl-4-(3-hydroxyphenyl)piperidine

  • Product Description

  • Seller Information

  • Description

    Synonym(s):3,3’,5,5’-Tetramethylbenzidine
    Empirical Formula:C16H20N2
    CAS Number:54827-17-7
    EC Number:259-364-6
    Molecular Weight:240.35
    Assay:≥99.0%
    Catalog Number:S0025

    Shelf Life/Retest:Two years


    ANALYSIS

    SPECIFICATIONS

    Appearance

    White/ light yellow crystal

    Purity (%)

    ≥99.0

    MP ,°C

    168.0-171.0

    Thin layer chromatography (TLC)

    Single spot

    Heavy Metal, ppm

    ≤5

    Loss on drying, %

    ≤0.5


    Description
    3,3’,5,5’-Tetramethylbenzidine (TMB) is a chemical compound with a molecular formula C16H20N2. This organic compound is widely utilized in biochemistry and molecular biology as a substrate for the detection of peroxidase activity. TMB undergoes colorimetric changes in the presence of peroxidase, resulting in the development of a blue color. This makes it a crucial component in various enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry applications.    

    Application    
    TMB's distinctive color reaction is employed to visualize and quantify the presence of specific biological molecules, such as antigens or antibodies, in various assays. The color change occurs due to the oxidation of TMB by peroxidase, leading to the formation of a blue product. The intensity of the color is directly proportional to the amount of peroxidase activity, providing a sensitive and quantitative measure for detecting biological interactions.

    Beyond its applications in the field of life sciences, 3,3’,5,5’-Tetramethylbenzidine finds use in other areas, such as analytical chemistry, where it serves as a reagent for the detection of various substances. Its versatility, stability, and reliable colorimetric response make TMB a valuable tool in research and diagnostic settings, contributing to advancements in our understanding of biological processes and disease detection.

    A TMB stock solution can be prepared by dissolving 1 mg/ml in DMSO. The working solution is made by diluting 1 ml of the DMSO stock with 9 ml phosphate citrate buffer, pH 5.0, and then adding 2 ml of fresh 30%
    hydrogen peroxide per 10 ml of substrate/buffer solution immediately prior to use. Stock solutions of TMB in DMSO can be frozen in 1–2 ml aliquots at –20 °C. Solutions appear to remain active for at least two years when stored in this manner. Any oxidation would be revealed as a color change in
    the working solution.

    Package Size    
    500g/bottle, 1kg/bottle, 25kg/drum

    Storage/Shipping    
    2-8ºC in dark

    Basic Info
    Product Name:

    3,3′,5,5′-Tetramethylbenzidine

    Other Name:

    [1,1′-Biphenyl]-4,4′-diamine,3,3′,5,5′-tetramethyl-;Benzidine,3,3′,5,5′-tetramethyl-;3,3′,5,5′-Tetramethyl[1,1′-biphenyl]-4,4′-diamine;3,3′,5,5′-Tetramethylbenzidine;3,5,3′,5′-Tetramethylbenzidine;TMB;BM blue;TMB substrate;3,3′,5,5′-Tetramethylbiphenyl-4,4′-diamine;K-Blue Max;ColorBurst Blue;TMB Blotting Plus;Enhanced K-Blue;Sure Blue TMB;K-Blue;3,3′,5,5′-Tetramethyl-4,4′-diaminobiphenyl

    CAS No.:

    54827-17-7

    Molecular Formula:

    C16H20N2

    InChIKeys:

    InChIKey=UAIUNKRWKOVEES-UHFFFAOYSA-N

    Molecular Weight:

    240.34300

    Exact Mass:

    240.34

    EC Number:

    259-364-6

    UNII:

    3B3T5CB8EO

    DSSTox ID:

    DTXSID5026120

    HScode:

    29215990

    Categories:

    Organic Reagents

    Characteristics
    PSA:

    52.04000

    XLogP3:

    3.6

    Appearance:

    White or light yellow solid

    Density:

    1.071 g/cm3

    Melting Point:

    168.5 °C

    Boiling Point:

    368.6ºC at 760 mmHg

    Flash Point:

    210.8±26.0 °C

    Refractive Index:

    1.594

    Water Solubility:

    H2O: Slightly soluble .

    Storage Conditions:

    2-8ºC

    Air and Water Reactions:

    Insoluble in water.

    Reactive Group:

    Amines, Aromatic

    Reactivity Profile:

    3,3',5,5'-TETRAMETHYLBENZIDINE is sensitive to prolonged exposure to light (NTP, 1992). Neutralizes acids in exothermic reactions to form salts plus water. May be incompatible with isocyanates, halogenated organics, peroxides, phenols (acidic), epoxides, anhydrides, and acid halides. Flammable gaseous hydrogen may be generated in combination with strong reducing agents, such as hydrides.

    Hazard Identification

    Classification of the substance or mixture

    Skin irritation, Category 2

    Eye irritation, Category 2

    Specific target organ toxicity – single exposure, Category 3

    GHS label elements, including precautionary statements

    Pictogram(s)
    Signal word

    Warning

    Hazard statement(s)

    H315 Causes skin irritation

    H319 Causes serious eye irritation

    H335 May cause respiratory irritation

    Precautionary statement(s)
    Prevention

    P264 Wash ... thoroughly after handling.

    P280 Wear protective gloves/protective clothing/eye protection/face protection/hearing protection/...

    P261 Avoid breathing dust/fume/gas/mist/vapours/spray.

    P271 Use only outdoors or in a well-ventilated area.

    Response

    P302+P352 IF ON SKIN: Wash with plenty of water/...

    P321 Specific treatment (see ... on this label).

    P332+P317 If skin irritation occurs: Get medical help.

    P362+P364 Take off contaminated clothing and wash it before reuse.

    P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

    P304+P340 IF INHALED: Remove person to fresh air and keep comfortable for breathing.

    P319 Get medical help if you feel unwell.

    Storage

    P403+P233 Store in a well-ventilated place. Keep container tightly closed.

    P405 Store locked up.

    Disposal

    P501 Dispose of contents/container to an appropriate treatment and disposal facility in accordance with applicable laws and regulations, and product characteristics at time of disposal.

    Other hazards which do not result in classification

    no data available

    Handling and Storage

    Precautions for safe handling

    Handling in a well ventilated place. Wear suitable protective clothing. Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Use non-sparking tools. Prevent fire caused by electrostatic discharge steam.

    Conditions for safe storage, including any incompatibilities

    Store the container tightly closed in a dry, cool and well-ventilated place. Store apart from foodstuff containers or incompatible materials.

  • Seller Information
    Business Type:

    Manufactory

    Main Products:

    tris,EDOT,[2,2]Paracyclophane,Guanidine hydrochloride,Bovine albumin,(3R,4R)-3,4-Dimethyl-4-(3-hydroxyphenyl)piperidine

    Location:

    No.17Jinhai Rd, Suzhou Industrial Park, China

    Payment Terms:

    TT against copy of documents,D/P,L/C,D/A,O/A,TT against B/L draft before shipment,100% TT in advance

    Average lead Time:

    2

    Total Annual Revenue:

    $50 million-$100 million

    Total Employees:

    501-1000

    Year of Establishment:

    2003

    Suzhou yacoo science co.,LTD was founded in 2003. It is a high-tech enterprise which specializing in biomedicine, electrochemical materials, import and export business, electronic mall, Up to now,It has 10 years history on chemical sales, R & D and production operations. Company also set sales and service outlets in Shanghai, Beijing, Guangzhou, Jinan and other cities, which owning first-class marketing team and technical support personnel. Its famous brands-Yacoo were exported to Europe, America and Southeast Asian countries. In order to meet the demand of the market of High-tech industrial products which updates fast, respones service timely, requires high technical and grows rapidly , Yacoo has established 5000㎡ research and test center and 10000㎡ production base, which have passed ISO9001: 2008 quality system certification.Yacoo corporation provides more than 20000 kinds of products of scientific research for Chinese and global customers with rapid logistics network, quality of service. At the same time ,it also provides customers with research and custom synthesis products from grams to tons,which relying on its own unique synthesis technology and rich R & D experience。The E-commerce site "Elements Mall" (www.b2star.com) belonging to Yacoo was formally operated in September 2012, which integrating domestic and international quality supplier of channel, and has realized one-stop shopping and services of the reagents and instruments, we dedicat to building a comprehensive professional services platform for Chinese scientists and echnology enterprises。Yacoo always adheres to customer demand-oriented, adhering to the continuous innovation as the support, adhering to provide quality products and technical solutions to customers。Our goal is to create ourself to become the leading international supplier and service provider of biological medicine and chemical material.

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