IPTG / Isopropyl-β-D-thiogalatopyranoside

IPTG / Isopropyl-β-D-thiogalatopyranoside

Chinese aliases: Isopropyl-Beta-D-thiogalactoside; Isopropyl-B-D-thiogalactopyranoside; Isopropyl-β-D-thiogalactoside (IPTG); Isopropyl-β-D-thiogalactoside; Isopropylthio-β-D-galactoside (IPTG)

English name: Isopropyl-beta-D-thiogalactopyranoside

English abbreviation: IPTG

CAS Number: 367-93-1

Molecular formula: C9H18O5S

Molecular weight: 238.3

Appearance: white or almost white crystalline powder Purity: 98%-101%

Moisture: anhydrous, ≤0.2%

Solubility: soluble in water, methanol

PH value: 6.25

Melting point: 105°C-110°C

Specific rotation: -29.27

Density: 1.37g/cm3

Flash point: 219℃

Boiling point: 438.4℃ at 760 mmHg

Vapor pressure: 1.58E-09mmHg at 25℃

Specification: 1kg, 5kg, 25kg

Packing: 25kg/cardboard drum

Output: 1000 tons/year

Uses: Isopropyl-beta-D-thiogalactopyranoside [IPTG] is a very powerful inducer, which is not metabolized by bacteria and is very stable, so it is widely used in laboratories. IPTG is often used in cloning experiments that require the induction of β-galactosidase activity. It is often used in combination with X-Gal or Bluo-Gal for blue and white screening of recombinant bacterial colonies, which can induce the expression of the lac operon in E. coli. IPTG binds to the lacI repressor protein and changes its conformation to play a role, preventing the inhibition of the β-galactosidase encoding gene lacZ.

Applicable to: E. coli induced expression, enzyme protein induced expression, gene induced expression, biological fermentation, usually used with X-GAL [CAS: 7240-90-6] for the screening of blue and white colonies. When IPTG is used for inducing expression experiments, the products usually matched together are X-GAL, SDS (sodium dodecyl sulfate), odor phenol blue, DTT, glycerin, PMSF, etc.