MOTS-c (human) Lyophilized Powder, Research Grade Peptide, CAS 1627580-64-6

Product Introduction

• • 1. Application Scope & Preconditions

    
    
    This product is a professional research-grade mitochondrial-derived peptide material, only applicable to laboratory scientific research, in vitro cell experiments, exploration of mitochondrial functional mechanisms, metabolic pathway analysis and other basic academic research. It is prohibited for clinical treatment, human trial, food addition, cosmetic formulation and any other non-research industrial production activities. All operators shall possess basic experimental qualifications and be familiar with laboratory safety regulations. Before operation, please read the product description and compliance statement thoroughly to clarify the product nature and scope of application.
    
    

    2. Pre-experiment Preparation

    
    

    2.1 Preparation of Environment & Apparatus

    
    
    1. All operations must be conducted in a laboratory that meets cleanliness standards. A biosafety cabinet or clean bench is recommended. Turn on relevant equipment 30 minutes in advance to purify the operating space and prevent microbial and particulate contamination of samples.
    2. Prepare sterile laboratory tools including sealed vials, pipettes, sterile tips, centrifuge tubes, glass rods, sealing films, alcohol swabs, disposable sterile gloves, dust-proof masks and goggles. All utensils shall be sterilized and dried before use.
    3. Pre-calibrate experimental devices such as centrifuges, constant temperature shakers, cell incubators and pH detectors to ensure stable operation and accurate parameters for follow-up experiments.
    
    

    2.2 Reagent & Sample Pre-treatment

    
    
    1. Solvent selection: Use sterile purified water, normal saline or suitable laboratory buffer solutions as the dissolving medium. Preheat the solvent to room temperature (20°C-25°C) to avoid damage to peptide activity caused by low temperature.
    2. Sample tempering: Take the vial of MOTS-c lyophilized powder out from -20°C storage and place it at room temperature for 15 to 20 minutes to balance the internal temperature, so as to avoid excessive foam and powder adhesion during dissolution due to temperature difference.
    3. Disinfection: Wipe the rubber stopper of the vial, pipette interfaces and tube openings with alcohol swabs, and wait until fully dry before use. Maintain strict aseptic operation throughout the whole process.
    
    

    3. Standard Dissolution Procedures

    
    
    1. Concentration calculation: Calculate the required solvent volume according to experimental design. For reference: dissolve 5mg powder in 0.5mL solvent, 10mg powder in 1mL solvent and 20mg powder in 2mL solvent to prepare the solution at the concentration of 10mg/mL. The concentration can be adjusted as required.
    2. Solvent addition: Draw the preheated solvent with a sterile pipette and add it slowly along the inner wall of the vial. Do not impact the powder directly to prevent excessive foam and structural damage of peptide molecules.
    3. Gentle dissolution: Tilt and rotate the vial slowly after adding solvent to fully soak and dissolve the powder. Severe shaking, swirling or high-speed vortexing are forbidden. Make sure the powder is completely dissolved to form a clear solution without sediment or suspended matters.
    4. Solution transfer: Transfer the prepared solution into pre-prepared sterile centrifuge tubes. Divide the liquid into small tubes according to single experimental dosage to reduce repeated freezing and thawing and maintain solution stability.
    5. Marking: Clearly label the product name, concentration, preparation date, operator and valid period on each container for easy use and traceability.
    
    

    4. Application Methods for Different Research Scenarios

    
    

    4.1 In Vitro Cell Experiments

    
    
    1. Cell pre-cultivation: Culture target cells until they reach the logarithmic growth phase with confluence of 70% to 80%, then replace with fresh culture medium for standby use.
    2. Concentration gradient setting: Dilute the stock solution to target working concentration with cell culture medium. The commonly used concentration range is 0.1μM to 10μM.
    3. Sample treatment: Add the diluted working solution into cell culture plates or flasks gently, then place the samples in a 37°C incubator with 5% CO₂. The incubation duration ranges from 24 hours to 72 hours based on research purposes.
    4. Subsequent detection: After incubation, conduct tests including cell viability detection, mitochondrial membrane potential measurement, oxidative stress index analysis, Western Blot and immunofluorescence staining to explore the effects of MOTS-c on cell metabolism, mitochondrial functions and signaling pathways.
    
    

    4.2 Mitochondrial Function Research

    
    
    1. Mitochondria extraction: Isolate complete mitochondria from fresh tissues or cells via differential centrifugation. All operations shall be performed at 4°C to maintain the integrity and activity of mitochondria.
    2. Reaction system preparation: Add appropriate amount of MOTS-c solution to the mitochondrial reaction buffer, with final concentration ranging from 1μM to 5μM. Set up blank control groups and negative control groups to eliminate interference factors.
    3. Functional detection: Detect the activity of mitochondrial respiratory chain, ATP production, reactive oxygen species level and calcium homeostasis by using fluorescence spectrophotometer, chemiluminescence detector and other devices, so as to analyze the impact of MOTS-c on physiological functions of mitochondria.
    
    

    4.3 Research on Metabolic Mechanisms & Molecular Pathways

    
    
    1. Sample treatment: Extract total protein, total RNA or metabolites from cells and tissues after intervention with MOTS-c for subsequent molecular biological tests.
    2. Index detection: Detect the mRNA expression level of metabolism-related genes via qRT-PCR, test protein expression and phosphorylation level by Western Blot, and analyze the content changes of carbohydrates, lipids, amino acids and other metabolites through metabolomics technology, so as to explore the molecular mechanism of MOTS-c in metabolic regulation.
    
    

    5. Post-use Treatment & Notes

    
    

    5.1 Storage of Residual Solution

    
    
    1. Seal the remaining stock solution and sub-packaged solution tightly immediately after use. Store them at 2°C to 8°C away from light, and use up within one month to avoid activity decline and contamination.
    2. Repeated freezing and thawing of diluted working solution are prohibited. Prepare the solution according to actual experimental dosage every time, and do not reuse the leftover working solution to avoid experimental errors.
    
    

    5.2 Safety Protection & Waste Disposal

    
    
    1. Wear gloves, goggles and masks during the whole operation. Avoid direct contact of skin and mucous membranes with powder or solution. In case of accidental contact, rinse thoroughly with plenty of running water and seek medical treatment when necessary. Ingestion and inhalation of this product are strictly forbidden.
    2. Clean and disinfect the operating platform with 75% alcohol after experiments. Collect used pipette tips, centrifuge tubes, gloves and other consumables separately. Dispose of all experimental wastes in accordance with laboratory hazardous waste management rules and local environmental protection regulations.
    
    

    5.3 Important Reminders

    
    
    1. Do not use the solution if it turns turbid, precipitates, discolors or develops peculiar smell. Prepare new solution instead. It is recommended to use products of the same batch for a complete set of experiments to ensure repeatability.
    2. Strictly control the environmental temperature, humidity and aseptic conditions during experiments. Keep the product away from strong light and high temperature to prevent loss of peptide activity.
    3. This product is only a raw material for scientific research. All experimental data are for academic reference only and shall not be directly applied to human-related research. Please indicate the product attribute and research purpose when publishing relevant research results.

• 1. • Please always keep the original container fully sealed to block outside air, moisture and dust, which can effectively protect the inherent physical and chemical properties of the product and prevent quality deterioration during storage.
       
       
       For long-term preservation of unopened products, place the sealed vials in a dry, cool and completely dark storage area at a constant temperature of -20°C. Keep the goods far away from high-temperature zones, direct strong light and humid surroundings. Frequent temperature changes and repeated freezing and thawing must be avoided, as such improper operations may damage the molecular structure of the peptide and reduce the stability of the material.
       
       
       Once the original packaging is opened for sampling and use, please seal the container tightly right away. Store the remaining materials under refrigeration at a temperature between 2°C and 8°C, and it is strongly recommended to consume all leftover materials within one month to guarantee stable performance for subsequent experiments.
       
       
       This product shall be stored separately from other types of chemical reagents, laboratory solvents and experimental raw materials. Do not place them together, so as to prevent cross-contamination between different substances. Please arrange regular inspection during the storage period. If any abnormal conditions such as agglomeration or discoloration are found, stop using the product immediately. All storage work must follow laboratory safety management specifications and relevant local regulations.

Core Business & Product Portfolio

• Therapeutic peptides: SS-31 (Elamipretide), Tirzepatide, Semaglutide, Retatrutide (RT20), etc.
• Functional peptides: GHK-Cu, TB500, KPV, NAD, Glutathione, etc.
• Custom peptide services: Custom synthesis of complex sequences, long-chain peptides (up to 200 amino acids) and cyclic peptides, as well as various modification services (N-methylation, unusual amino acid incorporation, etc.).

R&D & Manufacturing Strength

• Technical expertise: Mastery of core technologies including solid-phase peptide synthesis (SPPS), liquid-phase synthesis, advanced purification (HPLC) and strict structural characterization.
• Quality assurance: All products are produced in compliance with cGMP standards, with complete CoA, HPLC and MS reports to ensure batch-to-batch consistency and high purity (≥98%).
• Production capacity: Equipped with professional production bases in Guangzhou, supporting small-batch R&D samples to large-scale commercial production, with stable supply capacity.

Global Market & Service Philosophy

Vision