98% 3-Aminophthalhydrazide/ Luminol Powder CAS 521-31-3

1. Used as chemical analysis reagent and indicator.

2. Chemiluminescence analysis detection reagent (e.g. determination of metal cation or blood)

3. For chemiluminescence analysis, such as metal cation, blood and glucocorticoid

4. Luminescence test: Emmax 440 nm (chemiluminescence; 60 mM K2S2O8, 100 mM K2CO3, pH 11.5; Chemiluminescence reagents and indicators after the addition of H2O2 are often used for chemiluminescence analysis, such as metal cation, blood immunity and so on.

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Chemilum inescence, as a sensitive measurement method, is widely used to detect free radicals and reaction metabolites generated in enzymes, cells and biological organisms. The light of oxidation products and metabolites can be detected by various photometers. Chemiluminescence is widely used in the screening and study of antioxidants, such as polyphenols, polysaccharides, flavonoids and anthraquinones, due to its sensitive, rapid, simple operation and low price. The chemiluminescence systems commonly used for the determination of superoxide anions are xanthine oxidase -luminol, phloroglucinol and dimethyl sulfoxide -luminol. The former belongs to the enzymatic system, while the latter two belong to the non-enzymatic system. The chemiluminescence system of hydroxyl radicals are mainly copper sulfate - yeast (or bone marrow cells) - ascorbic acid and hydrogen peroxide, CuCl and H2O2 - adjacent to the Philippines then Lin - carbonate buffer, the copper sulfate - adjacent to the Philippines then Lin - ascorbic acid and hydrogen peroxide, ferrous sulfate and luminol hydrogen peroxide and ferrous sulfate - five luminol chemiluminescence system, are involved in the classic Fenton reaction to produce hydroxyl radicals, and then attack FaGuangJi produce chemiluminescence, can be used to measure the activity of extract to remove active oxygen free radicals.

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Method: the development of three phenol - luminol chemiluminescence system: luminol in 0.05 mol/L NaOH solution with a concentration of 0.05 mol/L solution, keep in dark place, before use with double evaporate water to dilute a tendency for 1 / L solution, the development of three phenol with a tendency for 0.01 mol/L solution made up L HCl, 4 ºC refrigerator save, before use water to steam with double dilution into 16 times 6.25 x 10-4 mol/L solution. 0.05mol/L pH10.2 na2co3-nahco3 buffer (containing 0.1mmol/L EDTA) was prepared before use, and was mixed with 1mmol/L rumino at 2:1 (volume fraction) before the experiment to form rumino and carbonate buffer mixture. Measurement, the luminous pool injection 10.0 mu of different concentrations of L (0,0.08, 0.4, 2, and 10 mg/mL) samples (as control) by sample buffer, then inject 6.25 x 10-4 mol/L phthalate three phenol 0.05 mL, add luminol and carbonate buffer mixture 0.94 mL start reaction (30 ºC), interval of 2 s counts the luminous intensity, determination of total luminous integral strength of the 300 s, the end of luminous intensity is not light value of the adjacent benzene third phenol.

In addition, there are chemical fluorescence method, fluorescence spectrometry and NBS- dichlorofluorescein chemiluminescence system for the determination of fluorescence chemiluminescence method, etc., their biggest advantage is high sensitivity.